Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.11 extracted from

  • Choi, M.Y.; Wang, Y.; Wong, L.L.; Lu, B.T.; Chen, W.Y.; Huang, J.D.; Tanner, J.A.; Watt, R.M.
    The two PPX-GppA homologues from Mycobacterium tuberculosis have distinct biochemical activities (2012), PLoS ONE, 7, e42561.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Guanosine 5'-pentaphosphate 1 mM, 73% resiudal activity Mycobacterium tuberculosis
guanosine 5'-tetraphosphate 1 mM, 68% resiudal activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0059
-
 (polyphosphate)14 pH 6.8, 37°C Mycobacterium tuberculosis
0.0121
-
 (polyphosphate)60 pH 6.8, 37°C Mycobacterium tuberculosis
0.0173
-
 (polyphosphate)130 pH 6.8, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ may partly substitute for Mn2+ Mycobacterium tuberculosis
Mn2+ required, optimum concentration 1 mM Mycobacterium tuberculosis
additional information no activity with Ca2+, Co2+, Cu2+ or Fe2+ ions Mycobacterium tuberculosis
Zn2+ may partly substitute for Mn2+ Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHV5
-
-
Mycobacterium tuberculosis H37Rv P9WHV5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)130 + H2O
-
 Mycobacterium tuberculosis (polyphosphate)129 + phosphate
-
 ?
(polyphosphate)130 + H2O
-
 Mycobacterium tuberculosis H37Rv (polyphosphate)129 + phosphate
-
 ?
(polyphosphate)14 + H2O
-
 Mycobacterium tuberculosis (polyphosphate)13 + phosphate
-
 ?
(polyphosphate)14 + H2O
-
 Mycobacterium tuberculosis H37Rv (polyphosphate)13 + phosphate
-
 ?
(polyphosphate)60 + H2O
-
 Mycobacterium tuberculosis (polyphosphate)59 + phosphate
-
 ?
(polyphosphate)60 + H2O
-
 Mycobacterium tuberculosis H37Rv (polyphosphate)59 + phosphate
-
 ?
additional information no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities Mycobacterium tuberculosis ?
-
 ?
additional information no substrate: guanosine pentaphosphate. Enzyme hydrolyzes ATP and ADP, but lacks GTPase activities Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
Rv0496
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.7
-
 (polyphosphate)130 pH 6.8, 37°C Mycobacterium tuberculosis
7.3
-
 (polyphosphate)60 pH 6.8, 37°C Mycobacterium tuberculosis
10.2
-
 (polyphosphate)14 pH 6.8, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
387
-
 (polyphosphate)130 pH 6.8, 37°C Mycobacterium tuberculosis
603
-
 (polyphosphate)60 pH 6.8, 37°C Mycobacterium tuberculosis
1729
-
 (polyphosphate)14 pH 6.8, 37°C Mycobacterium tuberculosis