Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium chloride | 200 mM, stimulates almost twofold in the presence and absence of 0.1 mM Co2+ | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | 42% of the activity in the lysate | Saccharomyces cerevisiae | 5829 | - |
vacuole | 20% of the activity in the lysate | Saccharomyces cerevisiae | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | best activator, maximum activation at 0.1 mM | Saccharomyces cerevisiae | |
Mg2+ | poor activation | Saccharomyces cerevisiae | |
Mn2+ | poor activition | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q04119 | bifunctional exo- and endopolyphosphatase, ECs 3.6.1.10 and 3.6.1.11 | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)15 + H2O | 88% of the activity with (phosphate)25 | Saccharomyces cerevisiae | (phosphate)14 + phosphate | - |
? | |
(phosphate)208 + H2O | 100% activity | Saccharomyces cerevisiae | (phosphate)207 + phosphate | - |
? | |
(phosphate)25 + H2O | 100% activity | Saccharomyces cerevisiae | (phosphate)24 + phosphate | - |
? | |
(phosphate)3 + H2O | 14% of the activity with (phosphate)25 | Saccharomyces cerevisiae | (phosphate)2 + phosphate | - |
? | |
(phosphate)45 + H2O | 100% activity | Saccharomyces cerevisiae | (phosphate)44 + phosphate | - |
? | |
(phosphate)9 + H2O | 45% of the activity with (phosphate)25 | Saccharomyces cerevisiae | (phosphate)8 + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Ppn1 | - |
Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
50% inhibition around 1 mg/L | Saccharomyces cerevisiae | heparin |
General Information | Comment | Organism |
---|---|---|
physiological function | strong constitutive overexpression of exopolyphosphatase PPN1 results in 28- and 11fold increase in activity compared to the PPN1 mutant and wild-type strains, respectively. The content of acid-soluble polyphosphate decreases about 6fold and the content of acid-insoluble polyphosphate decreases about 2.5fold in the cells of the transformant compared to the mutant strain | Saccharomyces cerevisiae |