Literature summary for 3.6.1.11 extracted from

Campos, E.; Facanha, A.R.; Costa, E.P.; da Silva Vaz, I.; Masuda, A.; Logullo, C.
Exopolyphosphatases in nuclear and mitochondrial fractions during embryogenesis of the hard tick Rhipicephalus (Boophilus) microplus (2008), Comp. Biochem. Physiol. B, 151, 311-316.

Search for more literature for 3.6.1.11

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	nuclear polyphosphates decrease and activity of exopolyphosphatase increases after embryo cellularization until the end of embryogenesis	Rhipicephalus microplus

Inhibitors

Inhibitors	Comment	Organism	Structure
heparin	heparin (0.02 mg/ml) inhibits nuclear and mitochondrial PPX activity in about 90 and 95%	Rhipicephalus microplus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0002	-	(phosphate)3	mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus
0.0007	-	(phosphate)3	nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus
0.0009	-	(phosphate)65	nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus
0.0011	-	(phosphate)15	nuclear fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus
0.0028	-	(phosphate)15	mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus
0.0036	-	(phosphate)65	mitochondrial fraction from eggs in the segmentation stage, in 50 mM Tris-HCl, 5 mM MgCl2, pH 7.5	Rhipicephalus microplus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rhipicephalus microplus	5739	-
additional information	existence of 2 different PPX isoforms operating in the nuclei and mitochondria with distinct metal dependence, inhibitor and activator sensitivities	Rhipicephalus microplus	-	-
nucleus	-	Rhipicephalus microplus	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	0.1 mM Co2+, PPX activity is stimulated by a factor of two in the nuclear fraction, but not in the mitochondrial fraction	Rhipicephalus microplus
Mg2+	2.5 mM Mg2+, PPX activity is stimulated by a factor of two in the nuclear fraction and in the mitochondrial fraction	Rhipicephalus microplus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(phosphate)n + H2O	Rhipicephalus microplus	regulatory enzyme in polyphosphate metabolism	(phosphate)n-1 + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhipicephalus microplus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
egg	segmentation stage, 9th day after oviposition	Rhipicephalus microplus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	increasing amounts of total RNA extracted from eggs progressively enhance nuclear PPX activity, whereas it exerts no effect on mitochondrial PPX activity, nuclear PPX activity increases throughout embryogenesis	Rhipicephalus microplus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(phosphate)15 + H2O	-	Rhipicephalus microplus	(phosphate)14 + phosphate	-	?
(phosphate)65 + H2O	-	Rhipicephalus microplus	(phosphate)64 + phosphate	-	?
(phosphate)n + H2O	regulatory enzyme in polyphosphate metabolism	Rhipicephalus microplus	(phosphate)n-1 + phosphate	-	?
triphosphate + H2O	-	Rhipicephalus microplus	diphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
exopolyphosphatase	-	Rhipicephalus microplus
polyphosphate-phosphohydrolase	-	Rhipicephalus microplus
PPX	-	Rhipicephalus microplus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Rhipicephalus microplus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Rhipicephalus microplus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)