Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.11 extracted from

  • Proudfoot, M.; Kuznetsova, E.; Brown, G.; Rao, N.N.; Kitagawa, M.; Mori, H.; Savchenko, A.; Yakunin, A.F.
    General Enzymatic Screens Identify Three New Nucleotidases in Escherichia coli. Biochemical Characterization of SurE, YfbR, and Yjjg (2004), J. Biol. Chem., 279, 54687-54694.
    View publication on PubMed
Search for more literature for 3.6.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

General Stability

General Stability Organism
stable Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Escherichia coli
CTP
-
 Escherichia coli
dATP
-
 Escherichia coli
dCTP
-
 Escherichia coli
dGTP
-
 Escherichia coli
dTTP
-
 Escherichia coli
GTP
-
 Escherichia coli
ITP
-
 Escherichia coli
TTP
-
 Escherichia coli
UTP
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 (Polyphosphate)n +/- 0.003 Escherichia coli
0.1
-
 3'-AMP +/- 0.01 Escherichia coli
0.26
-
 5'-GMP +/- 0.05 Escherichia coli
0.28
-
 5'-dGMP +/- 0.04 Escherichia coli
0.32
-
 5'-AMP +/- 0.04 Escherichia coli
0.37
-
 3'-CMP +/- 0.08 Escherichia coli
2.49
-
 p-nitrophenyl phosphate +/- 0.4 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ KD: 46.9 +/- 5.66 micorM Co2+ with p-nitrophenyl phosphate, KD: 10.7 +/- 1.07 microM Co2+ with 5'-AMP Escherichia coli
Mg2+ KD: 224.7 +/- 35.1 microM Mg2+ with p-nitrophenyl phosphate, KD: 140.0 +/- 9.99 microM Mg2+ with 5'-AMP Escherichia coli
Mn2+ KD: 7.24 +/- 0.71 microM Mn2+ with p-nitrophenyl phosphate, KD: 2.23 +/- 0.14 micorM Mn2+ with 5'-AMP Escherichia coli
Ni2+ KD: 72.3 +/- 7.61 microM Ni2+ with p-nitrophenyl phosphate, KD: 29.4 +/- 3.69 microM Ni2+ with 5'-AMP Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A840
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 +/- 0.01, polyphosphate Escherichia coli
7.24
-
 +/- 0.34, p-nitrophenyl phosphate Escherichia coli
10
-
 +/- 0.61, 5'-AMP Escherichia coli
12.1
-
 +/- 0.90, 3'-CMP Escherichia coli
16.4
-
 +/- 0.80, 5'-dGMP Escherichia coli
20.1
-
 +/- 0.76, 3'-AMP Escherichia coli
22.4
-
 +/- 1.78, 5'-GMP Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(polyphosphate)n + H2O
-
 Escherichia coli (polyphosphate)n-1 + phosphate
-
 ?
3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3'-CMP + H2O
-
 Escherichia coli cytosine + phosphate
-
 ?
5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer in solution Escherichia coli
oligomer at least four subunits in solution Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 (Polyphosphate)n +/- 0.005 Escherichia coli
3.55
-
 p-nitrophenyl phosphate +/- 0.16 Escherichia coli
4.9
-
 5'-AMP +/- 0.3 Escherichia coli
5.93
-
 3'-CMP +/- 0.44 Escherichia coli
8.04
-
 5'-dGMP +/- 0.39 Escherichia coli
9.84
-
 3'-AMP +/- 0.37 Escherichia coli
11
-
 5'-GMP +/- 0.87 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
24.1
-
 ATP with 3'-AMP as substrate Escherichia coli