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Literature summary for 3.6.1.1 extracted from

  • Kohn, G.; Delvaux, D.; Lakaye, B.; Servais, A.C.; Scholer, G.; Fillet, M.; Elias, B.; Derochette, J.M.; Crommen, J.; Wins, P.; Bettendorff, L.
    High inorganic triphosphatase activities in bacteria and mammalian cells: identification of the enzymes involved (2012), PLoS ONE, 7, e43879.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
diphosphate at pH 9.1 and 25°C Escherichia coli
0.91
-
Triphosphate at pH 9.1 and 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 20000, calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli
-
2 phosphate
-
?
diphosphate + H2O Escherichia coli MG1655
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MG1655
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
with triphosphate as substrate, at pH 9.1 and 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Escherichia coli 2 phosphate
-
?
diphosphate + H2O
-
Escherichia coli MG1655 2 phosphate
-
?
triphosphate + H2O the enzyme is less efficient for the hydrolysis of triphosphate than for diphosphate but is the main enzyme responsible for triphosphate hydrolysis in vivo Escherichia coli ?
-
?
triphosphate + H2O the enzyme is less efficient for the hydrolysis of triphosphate than for diphosphate but is the main enzyme responsible for triphosphate hydrolysis in vivo Escherichia coli MG1655 ?
-
?

Subunits

Subunits Comment Organism
? x * 20000, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Escherichia coli
PPase
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
the enzyme activity is remarkably heat-stable (up to 80°C) Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.7
-
Triphosphate at pH 9.1 and 25°C Escherichia coli
42
-
diphosphate at pH 9.1 and 25°C Escherichia coli