Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells as a fusion protein with a C-terminal polyhistidine tag | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
diphosphate | with 3 mM Mg2+, at pH 8.5 and 30°C | Trypanosoma cruzi | |
0.0167 | - |
Triphosphate | with 3 mM Co2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.0174 | - |
polyphosphate 75 | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.1554 | - |
guanosine 5'-tetraphosphate | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
acidocalcisome | - |
Trypanosoma cruzi | 20022 | - |
cytosol | - |
Trypanosoma cruzi | 5829 | - |
soluble | - |
Trypanosoma cruzi | - |
- |
vacuole | - |
Trypanosoma cruzi | 5773 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | preferred cofactor for hydrolysis of triphosphate | Trypanosoma cruzi | |
Mg2+ | preferred cofactor for the hydrolysis of diphosphate, highest stimulation of activity at about 0.5 mM | Trypanosoma cruzi | |
Zn2+ | preferred cofactor for hydrolysis of polyphosphate 75 | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, His-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
47900 | - |
x * 47900, calculated from amino acid sequence | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Trypanosoma cruzi | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
promastigote | - |
Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Trypanosoma cruzi | 2 phosphate | - |
? | |
guanosine 5'-tetraphosphate + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
polyphosphate 75 + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
triphosphate + H2O | - |
Trypanosoma cruzi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, His-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
? | x * 47900, calculated from amino acid sequence | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Trypanosoma cruzi |
PPase | - |
Trypanosoma cruzi |
VSP | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
for the hydrolysis of diphosphate | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000165 | - |
polyphosphate 75 | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.0022 | - |
guanosine 5'-tetraphosphate | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.13 | - |
Triphosphate | with 3 mM Co2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.22 | - |
diphosphate | with 3 mM Mg2+, at pH 8.5 and 30°C | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
for the hydrolysis of triphosphate or polyphosphate 75 | Trypanosoma cruzi |
8.5 | - |
for the hydrolysis of diphosphate | Trypanosoma cruzi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma cruzi | calculated from amino acid sequence | - |
6 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme-overexpressing parasites show a significant growth defect in fibroblasts, less responsiveness to hyperosmotic stress, and reduced persistence in tissues of mice | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00141 | - |
guanosine 5'-tetraphosphate | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
0.00948 | - |
polyphosphate 75 | with 3 mM Zn2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
7.8 | - |
Triphosphate | with 3 mM Co2+, at pH 6.5 and 30°C | Trypanosoma cruzi | |
27 | - |
diphosphate | with 3 mM Mg2+, at pH 8.5 and 30°C | Trypanosoma cruzi |