Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.1 extracted from

  • Galizzi, M.; Bustamante, J.M.; Fang, J.; Miranda, K.; Soares Medeiros, L.C.; Tarleton, R.L.; Docampo, R.
    Evidence for the role of vacuolar soluble pyrophosphatase and inorganic polyphosphate in Trypanosoma cruzi persistence (2013), Mol. Microbiol., 90, 699-715.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as a fusion protein with a C-terminal polyhistidine tag Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi
0.0167
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.0174
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.1554
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
acidocalcisome
-
Trypanosoma cruzi 20022
-
cytosol
-
Trypanosoma cruzi 5829
-
soluble
-
Trypanosoma cruzi
-
-
vacuole
-
Trypanosoma cruzi 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ preferred cofactor for hydrolysis of triphosphate Trypanosoma cruzi
Mg2+ preferred cofactor for the hydrolysis of diphosphate, highest stimulation of activity at about 0.5 mM Trypanosoma cruzi
Zn2+ preferred cofactor for hydrolysis of polyphosphate 75 Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, His-tagged enzyme, SDS-PAGE Trypanosoma cruzi
47900
-
x * 47900, calculated from amino acid sequence Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Trypanosoma cruzi
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
Trypanosoma cruzi
-
promastigote
-
Trypanosoma cruzi
-
trypomastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Trypanosoma cruzi 2 phosphate
-
?
guanosine 5'-tetraphosphate + H2O
-
Trypanosoma cruzi ?
-
?
polyphosphate 75 + H2O
-
Trypanosoma cruzi ?
-
?
triphosphate + H2O
-
Trypanosoma cruzi ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, His-tagged enzyme, SDS-PAGE Trypanosoma cruzi
? x * 47900, calculated from amino acid sequence Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
Trypanosoma cruzi
PPase
-
Trypanosoma cruzi
VSP
-
Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
for the hydrolysis of diphosphate Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000165
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.0022
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.13
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.22
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
for the hydrolysis of triphosphate or polyphosphate 75 Trypanosoma cruzi
8.5
-
for the hydrolysis of diphosphate Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi calculated from amino acid sequence
-
6

General Information

General Information Comment Organism
physiological function enzyme-overexpressing parasites show a significant growth defect in fibroblasts, less responsiveness to hyperosmotic stress, and reduced persistence in tissues of mice Trypanosoma cruzi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00141
-
guanosine 5'-tetraphosphate with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
0.00948
-
polyphosphate 75 with 3 mM Zn2+, at pH 6.5 and 30°C Trypanosoma cruzi
7.8
-
Triphosphate with 3 mM Co2+, at pH 6.5 and 30°C Trypanosoma cruzi
27
-
diphosphate with 3 mM Mg2+, at pH 8.5 and 30°C Trypanosoma cruzi