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Literature summary for 3.6.1.1 extracted from

  • Rodina, E.V.; Samygina, V.R.; Vorobyeva, N.N.; Sitnik, T.S.; Kurilova, S.A.; Nazarova, T.I.
    Structural and kinetic features of family I inorganic pyrophosphatase from Vibrio cholerae (2009), Biochemistry (Moscow), 74, 734-742.
    View publication on PubMed
Search for more literature for 3.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 15-20% (w7v) 2,4-methylpentanediol, and 3% (w/v) PEG4000 Vibrio cholerae serotype O1

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited by Na+ Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 diphosphate in the presence of 5 mM Mg2+, in 50 mM Tris-HCl, at pH 9.0 and 25°C Vibrio cholerae serotype O1
0.0028
-
 diphosphate in the presence of 0.005 mM Mn2+, in 50 mM HEPES-NaOH, at pH 7.5 and 25°C Vibrio cholerae serotype O1
0.004
-
 diphosphate in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH,at pH 7.5 and 25°C Vibrio cholerae serotype O1
0.0065
-
 diphosphate in the presence of Mg2+, at 25°C, pH not specified in the publication Vibrio cholerae serotype O1
0.007
-
 diphosphate in the presence of 10 mM Mg2+, in 50 mM Mes-NaOH, at pH 6.5 and 25°C Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Vibrio cholerae serotype O1
Mn2+ required for activity Vibrio cholerae serotype O1
additional information the enzyme retains measurable activity up to 0.7-0.8 M NaCl, KCl, or NH4Cl Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Vibrio cholerae serotype O1

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Vibrio cholerae serotype O1 phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Vibrio cholerae serotype O1

Synonyms

Synonyms Comment Organism
inorganic pyrophosphatase
-
 Vibrio cholerae serotype O1
PPase
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38
-
 diphosphate in the presence of 10 mM Mg2+, in 50 mM Mes-NaOH, at pH 6.5 and 25°C Vibrio cholerae serotype O1
51
-
 diphosphate in the presence of 0.005 mM Mn2+, in 50 mM HEPES-NaOH, at pH 7.5 and 25°C Vibrio cholerae serotype O1
93
-
 diphosphate in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH, at pH 7.5 and 25°C Vibrio cholerae serotype O1
177
-
 diphosphate in the presence of 5 mM Mg2+, in 50 mM Tris-HCl, at pH 9.0 and 25°C Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 in the presence of 5 mM Mg2+ Vibrio cholerae serotype O1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0026
-
 diphosphate in the presence of Mg2+, at pH 7.5 and 25°C Vibrio cholerae serotype O1