Activating Compound | Comment | Organism | Structure |
---|---|---|---|
magnesium methylenediphosphonate | a non-hydrolyzable analogue, the enzyme contains an extra binding site for the substrate magnesium diphosphate or its non-hydrolyzable analogue magnesium methylenediphosphonate, binding of substrate at the effector site of pyrophosphatase increases the rate of its hydrolysis at the active site, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D26A | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
D42E | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
E31A | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
K112Q | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
K115A | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
K148Q | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
R43Q | site-directed mutagenesis, the mutant shows a decrease in affinity to the effector site and, as a consequence, kinetics of substrate hydrolysis that do not obey the Michaelis-Menten equation | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis of wild-type and mutant enzymes with different substrates, detailed overview | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
lanthanum | results in a slow substrate binding to diphosphate | Escherichia coli | |
Mg2+ | dependent on, activates, bound to the substrate as magnesium diphosphate | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | - |
2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
82 | - |
0.0025 mM substrate Mg-diphosphate, in absence of magnesium methylenediphosphonate | Escherichia coli |
142 | - |
0.0025 mM substrate Mg-diphosphate, in presence of 0.0075 mM magnesium methylenediphosphonate | Escherichia coli |
360 | - |
0.015 mM substrate Mg-diphosphate, in presence or absence of 0.0075 mM magnesium methylenediphosphonate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
? | |
diphosphate + H2O | high activity with magnesium diphosphate, low activity with lanthanum diphosphate | Escherichia coli | 2 phosphate | - |
? | |
additional information | the enzyme contains an extra binding site for the substrate magnesium diphosphate or its non-hydrolyzable analogue magnesium methylenediphosphonate, binding of substrate at the effector site of pyrophosphatase increases the rate of its hydrolysis at the active site, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Escherichia coli |
PPase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |