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Literature summary for 3.6.1.1 extracted from
- Pyrophosphatase of the roundworm Ascaris suum plays an essential role in the worms molting and development (2005), Infect. Immun., 73, 1995-2004.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli | Ascaris suum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | PPase gene silencing by RNA interference, RNAi-mediated gene disruption suppressed native protein expression and mRNA levels in worm lung-stage larvae, recombinant AsPPase-immunized mice are protected from migration of challenge Ascaris suum larvae through the lungs | Ascaris suum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaF | - |
Ascaris suum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Ascaris suum | the enzyme plays an essential role in the worms molting and development, and in larval survival in the host, overview | 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography to near homogeneity | Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypodermis | lung-stage larvae | Ascaris suum | - |
| additional information | at various stages of development, the stages included were embryonated eggs, infective L3, lung-stage L3, lung-stage L3 that were further cultured in vitro for 5 days and removed after they had started molting to L4, this stage contained a mixed population of L3 and L4, and adult gravid female worms, highest activity at lung stage L3 cultured for 5 days, overview | Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
native activity at different developmental stages, highest activity at lung stage L3 cultured for 5 days, overview | Ascaris suum |
| 7.75 | - |
lung stage L3 cultured for 5 days | Ascaris suum |
| 940 | - |
purified recombinant His-tagged enzyme | Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Ascaris suum | 2 phosphate | - |
? | |
| diphosphate + H2O | the enzyme plays an essential role in the worms molting and development, and in larval survival in the host, overview | Ascaris suum | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | B-cell epitope mapping of the recombinant enzyme, epitopes are spread across the whole enzyme molecule | Ascaris suum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPase | - |
Ascaris suum |
| pyrophosphatase | - |
Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ascaris suum |
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