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Literature summary for 3.6.1.1 extracted from

  • Rantanen, M.K.; Lehtioe, L.; Rajagopal, L.; Rubens, C.E.; Goldman, A.
    Crystallization and preliminary crystallographic analysis of two Streptococcus agalactiae proteins: the family II inorganic pyrophosphatase and the serine/threonine phosphatase (2006), Acta Crystallogr. Sect. F, 62, 891-894.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Streptococcus agalactiae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, 5-20 mg/ml protein in 20 mM Tris-HCl, pH 7.0, hanging drop vapour diffusion method, 0.001 ml of protein and well solution are mixed, 2 weeks at room temperature, X-ray diffraction structure determination and analysis at 2.8 A resolution Streptococcus agalactiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on Streptococcus agalactiae

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae Q3K0B5
-
-
Streptococcus agalactiae A909 Q3K0B5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, removal of the tag by thrombin Streptococcus agalactiae

Synonyms

Synonyms Comment Organism
family II inorganic pyrophosphatase
-
Streptococcus agalactiae
manganese-dependent inorganic pyrophosphatase
-
Streptococcus agalactiae