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Literature summary for 3.6.1.1 extracted from
- Expression in Escherichia coli of the thermostable inorganic pyrophosphatase from the Aquifex aeolicus and purification and characterization of the recombinant enzyme (2001), Protein Expr. Purif., 23, 242-248.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Aquifex aeolicus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | cycle sequencing methods | Aquifex aeolicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli MV1184 | Aquifex aeolicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Aquifex aeolicus | |
| Cu2+ | - |
Aquifex aeolicus | |
| EDTA | - |
Aquifex aeolicus | |
| Hg2+ | - |
Aquifex aeolicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | minor activation | Aquifex aeolicus | |
| Mg2+ | required | Aquifex aeolicus | |
| Mn2+ | minor activation | Aquifex aeolicus | |
| Zn2+ | about the same activation as with Mg2+ | Aquifex aeolicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
gel filtration | Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Aquifex aeolicus | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O67501 | hyperthermophilic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Aquifex aeolicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.13 | - |
purified enzyme, pH 8.0, 80°C | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Aquifex aeolicus | 2 phosphate | - |
? | |
| diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions | Aquifex aeolicus | 2 phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 85 | - |
Aquifex aeolicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | - |
Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
50% of activity lost within 90 min | Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Aquifex aeolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Aquifex aeolicus |
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