Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.1 extracted from

  • Hyytiae, T.; Halonen, P.; Salminen, A.; Goldman, A.; Lahti, R.; Cooperman, B.S.
    Ligand binding sites in Escherichia coli inorganic pyrophosphatase: Effects of active site mutations (2001), Biochemistry, 40, 4645-4653.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D102V large decrease in metal binding affinity Escherichia coli
D65E large decrease in metal binding affinity Escherichia coli
D70E strongly decreased affinity for diphosphate Escherichia coli
D97E increases affinity for diphosphate Escherichia coli
E20D large decrease in metal binding affinity Escherichia coli
E20D strongly decreased affinity for diphosphate Escherichia coli
E31D increased metal binding affinity Escherichia coli
E31D strongly decreased affinity for diphosphate Escherichia coli
H136Q increased metal binding affinity Escherichia coli
K29R strongly decreased affinity for diphosphate Escherichia coli
additional information several mutants described that have minor effects on the binding of metal ions or diphosphate Escherichia coli
R43K strongly decreased affinity for diphosphate Escherichia coli
Y55F increased metal binding affinity Escherichia coli
Y55F strongly decreased affinity for diphosphate Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
hydroxymethylbisphosphonate competitive with diphosphate Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble type I Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, three binding sites in the absence of substrate Escherichia coli
Mn2+ can partially replace Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Escherichia coli important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Escherichia coli 2 phosphate
-
?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Escherichia coli 2 phosphate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.033
-
hydroxymethylbisphosphonate pH 7.2 Escherichia coli