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Literature summary for 3.6.1.1 extracted from

  • Hachimori, A.; Takeda, A.; Kaibuchi, M.; Ohkawara, N.; Samejima, T.
    Purification and characterization of inorganic pyrophosphatase from Bacillus stearothermophilus (1975), J. Biochem., 77, 1177-1183.
    View publication on PubMed
Search for more literature for 3.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Geobacillus stearothermophilus
NaF
-
 Geobacillus stearothermophilus
p-chloromercuribenzoate
-
 Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Geobacillus stearothermophilus
Mg2+
-
 Geobacillus stearothermophilus
Mn2+
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, SDS-PAGE Geobacillus stearothermophilus
122000
-
 ultracentrifuge method Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Geobacillus stearothermophilus
-
 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1800
-
-
 Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
0°C, 20 mM HCl-buffer, pH 8, for at least 2 months Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Geobacillus stearothermophilus 2 phosphate
-
 ?
diphosphate + H2O no other substrates Geobacillus stearothermophilus 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 no loss of activity within 2 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 10 in the presence of Mg2+ Geobacillus stearothermophilus
9.8
-
 in the presence of Mn2+ Geobacillus stearothermophilus