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Literature summary for 3.5.99.6 extracted from

  • Kwiatkowska-Semrau, K.; Czarnecka, J.; Wojciechowski, M.; Milewski, S.
    Heterogeneity of quaternary structure of glucosamine-6-phosphate deaminase from Giardia lamblia (2015), Parasitol. Res., 114, 175-184.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme activity is not modified by GlcNAc6P, UDPGlcNAc, or UDP-GalNAc Giardia intestinalis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Giardia intestinalis

General Stability

General Stability Organism
introduction of the oligoHis tag at the enzyme's C-terminus results in almost complete loss of the catalytic activity, while the catalytic properties of HisN-tagged enzyme and the untagged wild type are very similar Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21
-
alpha-D-glucosamine 6-phosphate HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
1.71
-
D-fructose 6-phosphate HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
2 3 NH3 untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
2.8
-
D-fructose 6-phosphate untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
3.52
-
D-fructose 6-phosphate HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
14
-
NH3 HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
38.1
-
alpha-D-glucosamine 6-phosphate untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
41.6
-
NH3 HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
45.2
-
alpha-D-glucosamine 6-phosphate HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30700
-
x * 30700, native enzyme, SDS-PAGE Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucosamine 6-phosphate + H2O Giardia intestinalis
-
D-fructose 6-phosphate + NH3
-
r
D-fructose 6-phosphate + NH3 Giardia intestinalis
-
alpha-D-glucosamine 6-phosphate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Resource Q column chromatography, and Ni2+-IDA agarose column chromatography Giardia intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
after 7.2fold purification, HisC-tagged enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
8.6
-
crude extract, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
62
-
after 7.2fold purification, untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis
73
-
after 7.2fold purification, HisN-tagged enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucosamine 6-phosphate + H2O
-
Giardia intestinalis D-fructose 6-phosphate + NH3
-
r
D-fructose 6-phosphate + NH3
-
Giardia intestinalis alpha-D-glucosamine 6-phosphate + H2O
-
r

Subunits

Subunits Comment Organism
? x * 30700, native enzyme, SDS-PAGE Giardia intestinalis
monomer or homodimer or homotetramer the recombinant and wild type enzyme exhibit heterogeneity of the quaternary structure and in solution exist in three interconvertible forms, namely, monomeric, homodimeric, and homotetrameric. The monomer/dimer/tetramer ratios depended on protein concentration and fall within the range from 72:27:1 to 39:23:38. The enzyme is fully active in each of the oligomeric structures Giardia intestinalis

Synonyms

Synonyms Comment Organism
2-amino-2-deoxy-D-glucose-6-phosphate aminohydrolase (ketol-isomerizing)
-
Giardia intestinalis
EC 5.3.1.10 formerly Giardia intestinalis
GlcN6P deaminase
-
Giardia intestinalis
glucosamine-6-phosphate deaminase
-
Giardia intestinalis
NagB
-
Giardia intestinalis