Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not modified by GlcNAc6P, UDPGlcNAc, or UDP-GalNAc | Giardia intestinalis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Giardia intestinalis |
General Stability | Organism |
---|---|
introduction of the oligoHis tag at the enzyme's C-terminus results in almost complete loss of the catalytic activity, while the catalytic properties of HisN-tagged enzyme and the untagged wild type are very similar | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.21 | - |
alpha-D-glucosamine 6-phosphate | HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
1.71 | - |
D-fructose 6-phosphate | HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
2 | 3 | NH3 | untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
2.8 | - |
D-fructose 6-phosphate | untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
3.52 | - |
D-fructose 6-phosphate | HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
14 | - |
NH3 | HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
38.1 | - |
alpha-D-glucosamine 6-phosphate | untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
41.6 | - |
NH3 | HisC-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis | |
45.2 | - |
alpha-D-glucosamine 6-phosphate | HisN-tagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30700 | - |
x * 30700, native enzyme, SDS-PAGE | Giardia intestinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucosamine 6-phosphate + H2O | Giardia intestinalis | - |
D-fructose 6-phosphate + NH3 | - |
r | |
D-fructose 6-phosphate + NH3 | Giardia intestinalis | - |
alpha-D-glucosamine 6-phosphate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Resource Q column chromatography, and Ni2+-IDA agarose column chromatography | Giardia intestinalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
after 7.2fold purification, HisC-tagged enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis |
8.6 | - |
crude extract, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis |
62 | - |
after 7.2fold purification, untagged wild type enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis |
73 | - |
after 7.2fold purification, HisN-tagged enzyme, with D-fructose 6-phosphate as substrate, at pH 7.0 and 37°C | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucosamine 6-phosphate + H2O | - |
Giardia intestinalis | D-fructose 6-phosphate + NH3 | - |
r | |
D-fructose 6-phosphate + NH3 | - |
Giardia intestinalis | alpha-D-glucosamine 6-phosphate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 30700, native enzyme, SDS-PAGE | Giardia intestinalis |
monomer or homodimer or homotetramer | the recombinant and wild type enzyme exhibit heterogeneity of the quaternary structure and in solution exist in three interconvertible forms, namely, monomeric, homodimeric, and homotetrameric. The monomer/dimer/tetramer ratios depended on protein concentration and fall within the range from 72:27:1 to 39:23:38. The enzyme is fully active in each of the oligomeric structures | Giardia intestinalis |
Synonyms | Comment | Organism |
---|---|---|
2-amino-2-deoxy-D-glucose-6-phosphate aminohydrolase (ketol-isomerizing) | - |
Giardia intestinalis |
EC 5.3.1.10 | formerly | Giardia intestinalis |
GlcN6P deaminase | - |
Giardia intestinalis |
glucosamine-6-phosphate deaminase | - |
Giardia intestinalis |
NagB | - |
Giardia intestinalis |