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Literature summary for 3.5.99.6 extracted from

  • Solar, T.; Tursic, J.; Legisa, M.
    The role of glucosamine-6-phosphate deaminase at the early stages of Aspergillus niger growth in a high-citric-acid-yielding medium (2008), Appl. Microbiol. Biotechnol., 78, 613-619.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-acetylglucosamine 6-phosphate activator of the enzyme in amination direction, leads to about 30% increased activity at 0.07-0.14 mM, overview Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
fructose 6-phosphate amination reaction, pH 8.0, 30°C Aspergillus niger
5
-
glucosamine 6-phosphate deamination reaction, pH 8.0, 30°C Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by ammonium ions at 5-20 mM Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucosamine 6-phosphate + H2O Aspergillus niger the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview fructose 6-phosphate + NH3
-
r
glucosamine 6-phosphate + H2O Aspergillus niger A158 the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview fructose 6-phosphate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger A158
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on a N-aminohexyl-glucosamine-6-phosphate resin folowed by dialysis and ultrafiltration Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.036
-
initial culture, cell homogenate Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosamine 6-phosphate + H2O
-
Aspergillus niger fructose 6-phosphate + NH3
-
r
glucosamine 6-phosphate + H2O the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview Aspergillus niger fructose 6-phosphate + NH3
-
r
glucosamine 6-phosphate + H2O
-
Aspergillus niger A158 fructose 6-phosphate + NH3
-
r
glucosamine 6-phosphate + H2O the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview Aspergillus niger A158 fructose 6-phosphate + NH3
-
r

Synonyms

Synonyms Comment Organism
GlcN6P deaminase
-
Aspergillus niger
glucosamine-6-phosphate isomerase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.3 70% of maximal activity at pH 6.8, maximal activity at pH 8.3 Aspergillus niger