Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-acetylglucosamine 6-phosphate | activator of the enzyme in amination direction, leads to about 30% increased activity at 0.07-0.14 mM, overview | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
fructose 6-phosphate | amination reaction, pH 8.0, 30°C | Aspergillus niger | |
5 | - |
glucosamine 6-phosphate | deamination reaction, pH 8.0, 30°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by ammonium ions at 5-20 mM | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosamine 6-phosphate + H2O | Aspergillus niger | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | fructose 6-phosphate + NH3 | - |
r | |
glucosamine 6-phosphate + H2O | Aspergillus niger A158 | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | fructose 6-phosphate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger A158 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on a N-aminohexyl-glucosamine-6-phosphate resin folowed by dialysis and ultrafiltration | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.036 | - |
initial culture, cell homogenate | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosamine 6-phosphate + H2O | - |
Aspergillus niger | fructose 6-phosphate + NH3 | - |
r | |
glucosamine 6-phosphate + H2O | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | Aspergillus niger | fructose 6-phosphate + NH3 | - |
r | |
glucosamine 6-phosphate + H2O | - |
Aspergillus niger A158 | fructose 6-phosphate + NH3 | - |
r | |
glucosamine 6-phosphate + H2O | the main enzyme in Aspergillus niger cells responsible for rapid glucosamine accumulation during the early stages of growth in a high-citric-acid-yielding medium, the enzyme must compete with 6-phosphofructo-1-kinase for the common substrate fructose 6-phosphate in Aspergillus niger cells, role of glucosamine in the pathway regulation, overview | Aspergillus niger A158 | fructose 6-phosphate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GlcN6P deaminase | - |
Aspergillus niger |
glucosamine-6-phosphate isomerase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.3 | 70% of maximal activity at pH 6.8, maximal activity at pH 8.3 | Aspergillus niger |