Cloned (Comment) | Organism |
---|---|
gene smu.636, cloning from genomic DNA, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, the protein solution contains 10 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, and equilibration against 0.5 ml of reservoir solution, hanging drop vapour diffusion method, X-ray diffraction structure determination and anaylsis at 2.4 A resolution | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
x * 25500, recombinant His-tagged enzyme, SDS-PAGE | Streptococcus mutans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosamine 6-phosphate + H2O | Streptococcus mutans | - |
fructose 6-phosphate + NH3 | - |
? | |
glucosamine 6-phosphate + H2O | Streptococcus mutans UA159 | - |
fructose 6-phosphate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
gene smu.636 | - |
Streptococcus mutans UA159 | - |
gene smu.636 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity in two steps by nickel affinity chromatography and gel filtration | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucosamine 6-phosphate + H2O | - |
Streptococcus mutans | fructose 6-phosphate + NH3 | - |
? | |
glucosamine 6-phosphate + H2O | - |
Streptococcus mutans UA159 | fructose 6-phosphate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25500, recombinant His-tagged enzyme, SDS-PAGE | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
NagB | - |
Streptococcus mutans |
SMU.636 protein | - |
Streptococcus mutans |