Literature summary for 3.5.99.6 extracted from

Altamirano, M.M.; Plumbridge, J.A.; Horjales, H.; Calcagno, M.L.
Asymmetric allosteric activation of Escherichia coli glucosamine-6-phosphate deaminase produced by replacement of Tyr121 (1995), Biochemistry, 34, 6074-6082.

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Protein Variants

Protein Variants	Comment	Organism
C228S	the kinetic and allosteric properties of the mutant enzyme in which Ser replaces Cys219 or Cys228 are the same as those described for the wild-type enzyme. The same result is obtained with the double mutation	Escherichia coli
Y121T	while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate	Escherichia coli
Y121W	while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.67	-	D-glucosamine 6-phosphate	pH 8.0, 30°C, mutant enzyme Tyr121Trp	Escherichia coli
2.01	-	D-glucosamine 6-phosphate	pH 8.0, 30°C, wild-type enzyme	Escherichia coli
2.6	-	D-glucosamine 6-phosphate	pH 8.0, 30°C, mutant enzyme Tyr121Thr	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucosamine 6-phosphate + H2O	-	Escherichia coli	D-fructose 6-phosphate + NH3	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Escherichia coli

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Release 2023.1 (January 2023)