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Literature summary for 3.5.99.5 extracted from

  • Colabroy, K.L.; Begley, T.P.
    Tryptophan catabolism: Identification and characterization of a new degradative pathway (2005), J. Bacteriol., 187, 7866-7869.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation new tryptophan catabolic pathway in Burholderia cepacia J2315, formation of the intermediate 4-oxalocrotonate differentiates this pathway from the proposed mammalian pathway which converts 2-aminomuconate to 2-ketoadipate and, ultimately, glutaryl-coenzyme A Burkholderia cepacia

Cloned(Commentary)

Cloned (Comment) Organism
cloned into plasmid pDESTF1 and overexpressed in Escherichia coli Tuner (DE3) Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
overexpressed soluble protein Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia J2315
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel-nitrilotriacetic acid affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate + H2O
-
Burkholderia cepacia 4-oxalocrotonate + NH3
-
?
2-aminomuconate + H2O
-
Burkholderia cepacia J2315 4-oxalocrotonate + NH3
-
?

Synonyms

Synonyms Comment Organism
2-aminomuconate deaminase
-
Burkholderia cepacia
AMD
-
Burkholderia cepacia