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Literature summary for 3.5.99.2 extracted from

  • Onozuka, M.; Konno, H.; Kawasaki, Y.; Akaji, K.; Nosaka, K.
    Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae (2008), FEMS Yeast Res., 8, 266-275.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O Saccharomyces cerevisiae physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08224 trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7
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Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
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?
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O hydrolyzed more than two times faster than thiamine Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3
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?
additional information Thi20p is involved in thiamine synthesis from pyrithiamine and oxythiamin Saccharomyces cerevisiae ?
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thiamine + H2O
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Saccharomyces cerevisiae 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
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?

Synonyms

Synonyms Comment Organism
Thi20p
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Saccharomyces cerevisiae
thiaminase II
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Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction thi20 deletion mutant cells can not grow in the presence of the synthetic thiamine antagonists, pyrithiamine and oxythiamin, indicating that the THI20 gene is necessary for the utilization of pyrithiamine and oxythiamine as precursors for thiamin Saccharomyces cerevisiae
physiological function thiaminase II activity of Thi20p is involved in the thiamine salvage pathway Saccharomyces cerevisiae