Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O | Saccharomyces cerevisiae | physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae | 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08224 | trifunctional enzyme: EC 3.5.99.2/EC 2.7.1.49/EC 2.7.4.7 | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O | physiological substrate, hydrolyzed more than two times faster than thiamine. Thiaminase II activity of Thi20p is involved in the thiamine salvage pathway by catalyzing the hydrolysis of 4-amino-5-hydroxymethyl-2-methylpyrimidine precursors in Saccharomyces cerevisiae | Saccharomyces cerevisiae | 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3 | - |
? | |
4-amino-5-aminomethyl-2-methyl-pyrimidine + H2O | hydrolyzed more than two times faster than thiamine | Saccharomyces cerevisiae | 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH3 | - |
? | |
additional information | Thi20p is involved in thiamine synthesis from pyrithiamine and oxythiamin | Saccharomyces cerevisiae | ? | - |
? | |
thiamine + H2O | - |
Saccharomyces cerevisiae | 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Thi20p | - |
Saccharomyces cerevisiae |
thiaminase II | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | thi20 deletion mutant cells can not grow in the presence of the synthetic thiamine antagonists, pyrithiamine and oxythiamin, indicating that the THI20 gene is necessary for the utilization of pyrithiamine and oxythiamine as precursors for thiamin | Saccharomyces cerevisiae |
physiological function | thiaminase II activity of Thi20p is involved in the thiamine salvage pathway | Saccharomyces cerevisiae |