Literature summary for 3.5.5.8 extracted from

Kataoka, S.; Arakawa, T.; Hori, S.; Katayama, Y.; Hara, Y.; Matsushita, Y.; Nakayama, H.; Yohda, M.; Nyunoya, H.; Dohmae, N.; Maeda, M.; Odaka, M.
Functional expression of thiocyanate hydrolase is promoted by its activator protein, P15K (2006), FEBS Lett., 580, 4667-4672.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	expression of thiocyanate hydrolase is promoted by its activator protein P15K	Thiobacillus thioparus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the alpha, beta, and gamma subunits in Escherichia coli, the subunits assemble to an apo-heterododecamer (alphabetagamma)4 like the wild-type enzyme exhibiting no catalytic activity due to lack of bound Co2+ irrespective of the cobalt concentration in the medium, coepression of enzyme with activator protein P15K leads to a functional Co2+-bound enzyme which exhibits 78% of native enzyme activity, optimization of conditions, overview	Thiobacillus thioparus

Cloned (Comment)

Organism

expression of the alpha, beta, and gamma subunits in Escherichia coli, the subunits assemble to an apo-heterododecamer (alphabetagamma)4 like the wild-type enzyme exhibiting no catalytic activity due to lack of bound Co2+ irrespective of the cobalt concentration in the medium, coepression of enzyme with activator protein P15K leads to a functional Co2+-bound enzyme which exhibits 78% of native enzyme activity, optimization of conditions, overview

Thiobacillus thioparus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	cobalt-containing metalloenzyme, 0.86 Co2+ per enzyme trimer, binding to the gamma-subunit requires the presence of activator protein P15K mediating the Co2+ incorporation, overview	Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
140000	-	native and apo-enzyme, gel filtration	Thiobacillus thioparus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
thiocyanate + H2O	Thiobacillus thioparus	-	carbonyl sulfide + NH3 + OH-	-	?
thiocyanate + H2O	Thiobacillus thioparus THI115	-	carbonyl sulfide + NH3 + OH-	-	?

Organism

Organism	UniProt	Comment	Textmining
Thiobacillus thioparus	-	-	-
Thiobacillus thioparus THI115	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from strain THI115, and recombinant enzyme from Echerichia coli strain BL21(DE3) by three steps of butyl-resin chromatography, hydroxyapatite and ion exchange chromatography, or by 4 steps of butyl-resin chromatography and gel filtration	Thiobacillus thioparus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
32	-	purified native enzyme	Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thiocyanate + H2O	-	Thiobacillus thioparus	carbonyl sulfide + NH3 + OH-	-	?
thiocyanate + H2O	-	Thiobacillus thioparus THI115	carbonyl sulfide + NH3 + OH-	-	?

Subunits

Subunits	Comment	Organism
dodecamer	heterododecamer (alphabetagamma)4 of heterotrimers	Thiobacillus thioparus

Synonyms

Synonyms	Comment	Organism
SCNase	-	Thiobacillus thioparus