KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
thiocyanate | - |
Thiobacillus thioparus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
23000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
32000 | - |
2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
126000 | - |
gel filtration | Thiobacillus thioparus |
142000 | - |
native PAGE | Thiobacillus thioparus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiocyanate + H2O | Thiobacillus thioparus | - |
carbonyl sulfide + NH3 + OH- | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacyl, hydroxylapatite | Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.3 | - |
- |
Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiocyanate + H2O | - |
Thiobacillus thioparus | carbonyl sulfide + NH3 + OH- | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 2 * 19000 + 2 * 23000 + 2 * 32000, deduced from amino acid sequence, SDS-PAGE | Thiobacillus thioparus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Thiobacillus thioparus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | enzyme shows high activity within this range | Thiobacillus thioparus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
treatment of the enzyme at 60°C for 5 min decreases activity below 10% of the original | Thiobacillus thioparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Thiobacillus thioparus |