Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 5% residual activity at 1 mM | uncultured bacterium | |
EDTA | 84% residual activity at 1 mM | uncultured bacterium | |
Fe2+ | 72% residual activity at 1 mM | uncultured bacterium | |
additional information | Nit1 is not or very weakly affected by Na+, Ca2+, Mg2+, and Fe3+ | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
glutaronitrile | at 30°C in 50 mM sodium phosphate buffer (pH 7.5) and 1 mM dithiothreitol | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | uncultured bacterium |
700000 | - |
gel filtration | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | E1A0Z9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylglutaronitrile + 2 H2O | 77% activity compared to glutaronitrile | uncultured bacterium | 4-cyanopentanoic acid + NH3 | - |
? | |
3-cyanopyridine + H2O | 11% activity compared to glutaronitrile | uncultured bacterium | 3-carboxypyridine + NH3 | - |
? | |
3-phenylpropionitrile + H2O | 59% activity compared to glutaronitrile | uncultured bacterium | ? | - |
? | |
acetonitrile + H2O | 5% activity compared to glutaronitrile | uncultured bacterium | ? | - |
? | |
adiponitrile + H2O | 91% activity compared to glutaronitrile | uncultured bacterium | adipate + NH3 | - |
? | |
butyronitrile + H2O | 6% activity compared to glutaronitrile | uncultured bacterium | ? | - |
? | |
cinnamonitrile + H2O | 28% activity compared to glutaronitrile | uncultured bacterium | cinnamate + NH3 | - |
? | |
crotonitrile + H2O | 7% activity compared to glutaronitrile | uncultured bacterium | ? | - |
? | |
glutaronitrile + H2O | 100% activity | uncultured bacterium | glutarate + NH3 | - |
? | |
additional information | Nit1 does not hydrolyze malononitrile, mandelonitrile, 4-cyanobenzoic acid, 3-ethoxybenzonitrile, 4-hydroxybenzonitrile, alpha-methylbenzyl cyanide, acrylonitrile, 4-tolunitrile, and benzonitrile | uncultured bacterium | ? | - |
? | |
additional information | Nit1 is an aliphatic nitrilase favoring dinitriles over mononitriles | uncultured bacterium | ? | - |
? | |
propionitrile + H2O | 5% activity compared to glutaronitrile | uncultured bacterium | ? | - |
? | |
succinonitrile + H2O | 76% activity compared to glutaronitrile | uncultured bacterium | succinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
NIT1 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
using the substrate glutaronitrile | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | up to 40°C Nit1 is stable for 1 h, while the loss of activity is about 50% after 30 min at 50°C, the enzyme is completely inactivated after 10 min incubation at 60°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
using the substrate glutaronitrile | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | Nit1 tolerates a pH range between 5 and 9 with an activity of more than 50% | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.483 | - |
glutaronitrile | at 30°C in 50 mM sodium phosphate buffer (pH 7.5) and 1 mM dithiothreitol | uncultured bacterium |