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Literature summary for 3.5.5.7 extracted from

  • Khandelwal, A.K.; Nigam, V.K.; Choudhury, B.; Mohan, M.K.; Ghosh, P.
    Optimization of nitrilase production from a new thermophilic isolate (2007), J. Chem. Technol. Biotechnol., 82, 646-651.
No PubMed abstract available
Search for more literature for 3.5.5.7

Activating Compound

Activating Compound Comment Organism Structure
Benzonitrile inducer of nitrilase production Streptomyces sp.
additional information there is no appreciable variation in the activity of enzyme at any concentration of glycerol Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. MTCC 7546
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylglutaronitrile + 4 H2O
-
 Streptomyces sp. 2-methylglutaric acid + 2 NH3
-
 ?
acetonitrile + H2O
-
 Streptomyces sp. acetic acid + NH3
-
 ?
acrylonitrile + H2O
-
 Streptomyces sp. acrylic acid + NH3
-
 ?
acrylonitrile + H2O
-
 Streptomyces sp. MTCC 7546 acrylic acid + NH3
-
 ?
adiponitrile + H2O
-
 Streptomyces sp. adipic acid + NH3
-
 ?
crotonitrile + H2O
-
 Streptomyces sp. crotonic acid + NH3
-
 ?
crotonitrile + H2O
-
 Streptomyces sp. MTCC 7546 crotonic acid + NH3
-
 ?
fumaronitrile + H2O
-
 Streptomyces sp. fumaric acid + NH3
-
 ?
fumaronitrile + H2O
-
 Streptomyces sp. MTCC 7546 fumaric acid + NH3
-
 ?
glutaronitrile + H2O
-
 Streptomyces sp. glutaric acid + NH3
-
 ?
hydrocinnamonitrile + H2O
-
 Streptomyces sp. hydrocinnamic acid + NH3
-
 ?
isobutyronitrile + H2O
-
 Streptomyces sp. isobutyrate + NH3
-
 ?
isovaleronitrile + H2O
-
 Streptomyces sp. isovaleric acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Streptomyces sp. mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Streptomyces sp. MTCC 7546 mandelic acid + NH3
-
 ?
additional information isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates Streptomyces sp. ?
-
 ?
additional information isovaleronitrile, benzonitrile, and pyridine-3-carbonitrile are weak substrates Streptomyces sp. MTCC 7546 ?
-
 ?
propionitrile + H2O
-
 Streptomyces sp. propionic acid + NH3
-
 ?
sebaconitrile + H2O
-
 Streptomyces sp. sebaconic acid + NH3
-
 ?
succinonitrile + H2O
-
 Streptomyces sp. succinic acid + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 50
-
 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.6 7.4
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6 7 enzymatic activity increases in the pH range 6.6 to 7.0 and then starts decreasing Streptomyces sp.