Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates at 1 mM | Pyrococcus abyssi | |
dithiothreitol | activates at 1 mM | Pyrococcus abyssi | |
dithiothreitol | 1 mM, 23% inhibition | Pyrococcus abyssi | |
ethylene glycol | 20% increase of activity at 10% ethylene glycol | Pyrococcus abyssi | |
L-cysteine | activates at 1 mM | Pyrococcus abyssi |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pyrococcus abyssi |
expression in Escherichia coli | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 29% inhibition | Pyrococcus abyssi | |
5,5'-dithiobis(2-nitrobenzoate) | complete inhibition at 5 mM | Pyrococcus abyssi | |
acetone | 10%, complete inhibition; complete inhibition at 10% acetone | Pyrococcus abyssi | |
Ag+ | 1 mM, complete inhibition; complete inhibition at 1 mM | Pyrococcus abyssi | |
Ca2+ | 1 mM, 20% inhibition; 75% residual activity at 5 mM | Pyrococcus abyssi | |
Cr3+ | 5 mM, 40% inhibition; 60% residual activity at 5 mM | Pyrococcus abyssi | |
dithiothreitol | 5 mM, 50% inhibition | Pyrococcus abyssi | |
DTNB | 5 mM, complete inhibition | Pyrococcus abyssi | |
ethanol | 57% inhibition at 30%, complete inhibition at 50%; complete inhibition at 50% ethanol | Pyrococcus abyssi | |
ethylene glycol | 80% inhibition at 50%; 80% inhibition at 50% ethylene glycol | Pyrococcus abyssi | |
Fe2+ | 40% residual activity at 5 mM; 5 mM, 60% inhibition | Pyrococcus abyssi | |
Hg2+ | 1 mM, complete inhibition; complete inhibition at 1 mM | Pyrococcus abyssi | |
iodacetamide | complete inhibition at 5 mM | Pyrococcus abyssi | |
iodacetate | complete inhibition at 5 mM | Pyrococcus abyssi | |
iodoacetamide | 5 mM, complete inhibition | Pyrococcus abyssi | |
iodoacetate | 5 mM, complete inhibition | Pyrococcus abyssi | |
L-Cys | 5 mM, 27% inhibition | Pyrococcus abyssi | |
Malononitrile | concentrations above 12 mM have a strong inhibitory effect; strong inhibition at 12 mM | Pyrococcus abyssi | |
methanol | complete inhibition at 30%; complete inhibition at 30% methanol | Pyrococcus abyssi | |
additional information | EDTA and NaN3 have no influence on activity | Pyrococcus abyssi | |
p-chloromercuribenzoate | complete inhibition at 1 mM | Pyrococcus abyssi | |
p-hydroxymercuribenzoate | 1 mM, complete inhibition; complete inhibition at 1 mM | Pyrococcus abyssi | |
PCMB | 1 mM, complete inhibition | Pyrococcus abyssi | |
phenyl hydrazine | 53% residual activity at 5 mM; 5 mM, 47% inhibition | Pyrococcus abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.47 | - |
Malononitrile | pH 7.4, 80°C | Pyrococcus abyssi | |
3.47 | - |
Malononitrile | in 20 mM potassium phosphate, pH 7.4, with 1 mM dithiothreitol, at 80°C | Pyrococcus abyssi | |
9.48 | - |
Fumaronitrile | pH 7.4, 80°C | Pyrococcus abyssi | |
9.48 | - |
Fumaronitrile | in 20 mM potassium phosphate, pH 7.4, with 1 mM dithiothreitol, at 80°C | Pyrococcus abyssi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pyrococcus abyssi | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29800 | - |
2 * 29800, gel filtration | Pyrococcus abyssi |
30000 | - |
2 * 30000, SDS-PAGE | Pyrococcus abyssi |
59800 | - |
gel filtration | Pyrococcus abyssi |
60000 | - |
gel filtration | Pyrococcus abyssi |
60000 | - |
SDS-PAGE | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Aeropyrum pernix | - |
- |
- |
no activity in Pyrococcus furiosus | - |
- |
- |
no activity in Pyrococcus horikoshii | - |
- |
- |
Pyrococcus abyssi | - |
- |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Q-Sepharose Fast Flow column chromatography and Sephadex PD-10 column gel filtration | Pyrococcus abyssi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00595 | - |
recombinant enzyme from crude extract | Pyrococcus abyssi |
0.1419 | - |
recombinant enzyme after 23.85fold purification | Pyrococcus abyssi |
141.9 | - |
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumaronitrile + H2O | - |
Pyrococcus abyssi | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | preferred substrate | Pyrococcus abyssi | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | fumaric acid + NH3 | - |
? | |
fumaronitrile + H2O | preferred substrate | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | fumaric acid + NH3 | - |
? | |
malononitrile + H2O | - |
Pyrococcus abyssi | malonic acid + NH3 | - |
? | |
malononitrile + H2O | preferred substrate | Pyrococcus abyssi | malonic acid + NH3 | - |
? | |
malononitrile + H2O | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | malonic acid + NH3 | - |
? | |
malononitrile + H2O | preferred substrate | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | malonic acid + NH3 | - |
? | |
additional information | compounds with a nitrile group bound to an aromatic ring or amino acid are not hydrolyzed by recombinant nitrilase | Pyrococcus abyssi | ? | - |
? | |
additional information | compounds with a nitrile group bound to an aromatic ring or amino acid are not hydrolyzed by recombinant nitrilase | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Pyrococcus abyssi |
homodimer | 2 * 30000, SDS-PAGE | Pyrococcus abyssi |
homodimer | 2 * 29800, gel filtration | Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pyrococcus abyssi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | - |
Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
25 h, recombinant enzyme loses about 50% of maximal activity | Pyrococcus abyssi |
70 | 90 | the recombinant enzyme is highly thermostable with a half-life of 25 h at 70°C, 9 h at 80°C, and 6 h at 90°C | Pyrococcus abyssi |
80 | - |
half-life: 9 h | Pyrococcus abyssi |
90 | - |
half-life: 6 h | Pyrococcus abyssi |
113 | - |
Tm-value: 112.7°C | Pyrococcus abyssi |
120 | - |
irreversible denaturation after heating to 120°C | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Pyrococcus abyssi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Pyrococcus abyssi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus abyssi | isoelectric focusing | - |
5.3 |