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Literature summary for 3.5.5.7 extracted from

  • Dhillon, J.; Chhatre, S.; Shanker, R.; Shivaraman, N.
    Transformation of aliphatic and aromatic nitriles by a nitrilase from Pseudomonas sp. (1999), Can. J. Microbiol., 45, 811-815.
No PubMed abstract available
Search for more literature for 3.5.5.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Pseudomonas sp.
0.75
-
 potassium cyanide pH 7.0, 37°C, partially purified enzyme Pseudomonas sp.
5.88
-
 acrylonitrile pH 7.0, 37°C, partially purified enzyme Pseudomonas sp.
10.28
-
 adiponitrile pH 7.0, 37°C, partially purified enzyme Pseudomonas sp.
11.26
-
 acetonitrile pH 7.0, 37°C, partially purified enzyme Pseudomonas sp.
12.27
-
 Benzonitrile pH 7.0, 37°C, partially purified enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 native PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 from soil
-
Pseudomonas sp. S1
-
 from soil
-

Purification (Commentary)

Purification (Comment) Organism
3-step procedure, partially, 10.9fold Pseudomonas sp.

Reaction

Reaction Comment Organism Reaction ID
R-CN + 2 H2O = R-COOH + NH3 preferentially hydrolyses aliphatic nitriles, some of which are apparently not substrates for other known nitrilases, EC 3.5.5.1. Substrates include crotonitrile, acrylnitrile and glutaronitrile Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.53
-
 partially purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetonitrile + H2O
-
 Pseudomonas sp. acetate + NH3
-
 ?
acetonitrile + H2O
-
 Pseudomonas sp. S1 acetate + NH3
-
 ?
acrylonitrile + H2O most effective substrate Pseudomonas sp. acrylate + NH3
-
 ?
adiponitrile + H2O
-
 Pseudomonas sp. adipate + NH3
-
 ?
benzonitrile + 2 H2O
-
 Pseudomonas sp. benzoic acid + NH3
-
 ?
benzonitrile + 2 H2O
-
 Pseudomonas sp. S1 benzoic acid + NH3
-
 ?
butyronitrile + H2O
-
 Pseudomonas sp. butyrate + NH3
-
 ?
butyronitrile + H2O
-
 Pseudomonas sp. S1 butyrate + NH3
-
 ?
glutaronitrile + H2O
-
 Pseudomonas sp. glutarate + NH3
-
 ?
glutaronitrile + H2O
-
 Pseudomonas sp. S1 glutarate + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Pseudomonas sp. phenylacetic acid + NH3
-
 ?
phenylacetonitrile + 2 H2O
-
 Pseudomonas sp. S1 phenylacetic acid + NH3
-
 ?
potassium cyanide + H2O
-
 Pseudomonas sp. ?
-
 ?
R-CN + H2O enzyme uses both aliphatic and aromatic nitriles, no formation of amide intermediate Pseudomonas sp. R-COOH + NH3
-
 ?
succinodinitrile + H2O
-
 Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
nitrilase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas sp.