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Literature summary for 3.5.5.5 extracted from

  • Bhatia, S.; Mehta, P.; Bhatia, R.; Bhalla, T.
    Purification and characterization of arylacetonitrile-specific nitrilase of Alcaligenes sp. MTCC 10675 (2014), Biotechnol. Appl. Biochem., 61, 459-465.
    View publication on PubMed

General Stability

General Stability Organism
in citrate buffer, the activity of the enzyme increases with the increase in the pH (4.0-5.5), whereas in borate and carbonate buffers very little activity is observed Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 50% inhibition at 1 mM Alcaligenes sp.
Cd2+ about 50% inhibition at 1 mM Alcaligenes sp.
Co2+ 77% inhibition at 1 mM Alcaligenes sp.
Cs+ about 50% inhibition at 1 mM Alcaligenes sp.
Cu2+ 87% inhibition at 1 mM Alcaligenes sp.
dithiothreitol about 50% inhibition at 1 mM Alcaligenes sp.
Hg2+ 98% inhibition at 1 mM Alcaligenes sp.
Mg2+ 91% inhibition at 1 mM Alcaligenes sp.
Mn2+ about 50% inhibition at 1 mM Alcaligenes sp.
additional information EDTA shows very little effect on arylacetonitrilase activity Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
13
-
mandelonitrile at pH 6.5 and 30°C Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Zn2+, Na+, and K+ show very little effect on arylacetonitrilase activity Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
8 * 60000, SDS-PAGE Alcaligenes sp.
520000
-
gel filtration Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. L7RY40
-
-
Alcaligenes sp. MTCC 10675 L7RY40
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphenylacetonitrile + H2O
-
Alcaligenes sp. diphenylacetic acid + NH3
-
?
diphenylacetonitrile + H2O
-
Alcaligenes sp. MTCC 10675 diphenylacetic acid + NH3
-
?
mandelonitrile + 2 H2O
-
Alcaligenes sp. mandelic acid + NH3
-
?
mandelonitrile + 2 H2O
-
Alcaligenes sp. MTCC 10675 mandelic acid + NH3
-
?
additional information the purified enzyme has very little activity against heterocyclic and aliphatic nitriles like 2-cyanopyridine and 4-cyanopyridine, and no activity is recorded against aromatic nitriles like benzonitrile, 4-hydroxynitrile, and tolunitrile Alcaligenes sp. ?
-
?
additional information the purified enzyme has very little activity against heterocyclic and aliphatic nitriles like 2-cyanopyridine and 4-cyanopyridine, and no activity is recorded against aromatic nitriles like benzonitrile, 4-hydroxynitrile, and tolunitrile Alcaligenes sp. MTCC 10675 ?
-
?
phenylacetonitrile + 2 H2O best substrate Alcaligenes sp. phenylacetic acid + NH3
-
?
phenylacetonitrile + 2 H2O best substrate Alcaligenes sp. MTCC 10675 phenylacetic acid + NH3
-
?

Subunits

Subunits Comment Organism
homooctamer 8 * 60000, SDS-PAGE Alcaligenes sp.

Synonyms

Synonyms Comment Organism
arylacetonitrile-hydrolyzing nitrilase
-
Alcaligenes sp.
arylacetonitrile-specific nitrilase
-
Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 55 exposure of the enzyme at 15, 25, and 35°C for 5 h causes 8%, 15%, and 28% decrease in arylacetonitrilase activity, respectively. A further increase in the temperature to 45 and 55°C results in 58% and 90% decrease in activity, respectively. The half-life of this enzyme at 15, 25, 35, 45, 50, and 55°C is 31 h, 16 h, 9 h, 4 h 30 min, 3 h 20 min, and 2 h 40 min, respectively Alcaligenes sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26
-
mandelonitrile at pH 6.5 and 50°C Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Alcaligenes sp.