Literature summary for 3.5.5.1 extracted from

Rustler, S.; Mueller, A.; Windeisen, V.; Chmura, A.; Fernandes, B.C.; Kiziak, C.; Stolz, A.
Conversion of mandelonitrile and phenylglycinenitrile by recombinant E. coli cells synthesizing a nitrilase from Pseudomonas fluorescens EBC191 (2007), Enzyme Microb. Technol., 40, 598-606.

No PubMed abstract available

Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain JM109(pIK9)	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-
Pseudomonas fluorescens EBC191	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
mandelonitrile + 2 H2O	-	Pseudomonas fluorescens	mandelic acid + NH3	-	?
mandelonitrile + 2 H2O	-	Pseudomonas fluorescens EBC191	mandelic acid + NH3	-	?
phenylglycinonitrile + H2O	-	Pseudomonas fluorescens	phenylglycine + NH3	-	?
phenylglycinonitrile + H2O	-	Pseudomonas fluorescens EBC191	phenylglycine + NH3	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)