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Literature summary for 3.5.5.1 extracted from

  • Wu, S.; Fogiel, A.J.; Petrillo, K.L.; Hann, E.C.; Mersinger, L.J.; DiCosimo, R.; OKeefe, D.P.; Ben-Bassat, A.; Payne, M.S.
    Protein engineering of Acidovorax facilis 72W nitrilase for bioprocess development (2007), Biotechnol. Bioeng., 97, 689-693.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Acidovorax facilis

Protein Variants

Protein Variants Comment Organism
F168K 2.5fold improved specific activity Acidovorax facilis
F168L 2.4fold improved specific activity Acidovorax facilis
F168V 2.5fold improved specific activity Acidovorax facilis
F174I 1.8fold improved specific activity Acidovorax facilis
T210A 7.3fold improved specific activity Acidovorax facilis
T210I 1.8fold improved specific activity Acidovorax facilis
Y65C 1.8fold improved specific activity Acidovorax facilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 SDS-PAGE Acidovorax facilis

Organism

Organism UniProt Comment Textmining
Acidovorax facilis
-
-
-
Acidovorax facilis 72W
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyvaleronitrile + H2O
-
 Acidovorax facilis 3-hydroxyvaleric acid + NH3
-
 ?
3-hydroxyvaleronitrile + H2O
-
 Acidovorax facilis 72W 3-hydroxyvaleric acid + NH3
-
 ?