Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)plys and BW310 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
head-to-tail dimer of the A3G catalytic deamination domain A3G-CD2, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate, pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) polyethylene glycol 4000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D264A | the variant has 5% of the catalytic efficiency of the wild type protein | Homo sapiens |
D370A | the variant has 16% of the catalytic efficiency of the wild type protein | Homo sapiens |
F268A | the variant has 25% of the catalytic efficiency of the wild type protein | Homo sapiens |
H248G | the variant has 158% of the catalytic efficiency of the wild type protein | Homo sapiens |
H250A | the variant has 266% of the catalytic efficiency of the wild type protein | Homo sapiens |
P210A | the variant has 1% of the catalytic efficiency of the wild type protein | Homo sapiens |
P210G | the variant has 10% of the catalytic efficiency of the wild type protein | Homo sapiens |
Q245A | the mutation nearly abolishes the catalytic efficiency to 5% compared to the wild type protein | Homo sapiens |
Q380A | the variant has 68% of the catalytic efficiency of the wild type protein | Homo sapiens |
R256A | the mutation nearly abolishes the catalytic efficiency to 1% compared to the wild type protein | Homo sapiens |
R374A | the variant has 3% of the catalytic efficiency of the wild type protein | Homo sapiens |
R376A | the variant has 15% of the catalytic efficiency of the wild type protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01615 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
0.01846 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
0.02042 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.02252 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
0.02705 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
0.03306 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
0.03325 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens | |
0.06323 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
0.08188 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
0.1189 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
0.1639 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
0.2785 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
0.3944 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | Homo sapiens | - |
uracil in single-stranded viral DNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HC16 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | - |
Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A3G | - |
Homo sapiens |
APOBEC3G | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000083 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
0.000102 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
0.000165 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
0.00017 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
0.0002 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
0.000517 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
0.00085 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
0.001 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
0.0014 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
0.0018 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.002 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens | |
0.0047 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
0.008 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210A, at pH 7.5 and 37°C | Homo sapiens | |
0.0012 | - |
cytosine in single-stranded viral DNA | mutant enzyme R256A, at pH 7.5 and 37°C | Homo sapiens | |
0.0031 | - |
cytosine in single-stranded viral DNA | mutant enzyme R374A, at pH 7.5 and 37°C | Homo sapiens | |
0.0046 | - |
cytosine in single-stranded viral DNA | mutant enzyme D264A, at pH 7.5 and 37°C | Homo sapiens | |
0.005 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q245A, at pH 7.5 and 37°C | Homo sapiens | |
0.009 | - |
cytosine in single-stranded viral DNA | mutant enzyme P210G, at pH 7.5 and 37°C | Homo sapiens | |
0.0135 | - |
cytosine in single-stranded viral DNA | mutant enzyme R376A, at pH 7.5 and 37°C | Homo sapiens | |
0.01425 | - |
cytosine in single-stranded viral DNA | mutant enzyme H248G, at pH 7.5 and 37°C | Homo sapiens | |
0.0143 | - |
cytosine in single-stranded viral DNA | mutant enzyme D370A, at pH 7.5 and 37°C | Homo sapiens | |
0.023 | - |
cytosine in single-stranded viral DNA | mutant enzyme F268A, at pH 7.5 and 37°C | Homo sapiens | |
0.062 | - |
cytosine in single-stranded viral DNA | mutant enzyme Q380A, at pH 7.5 and 37°C | Homo sapiens | |
0.09 | - |
cytosine in single-stranded viral DNA | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.24 | - |
cytosine in single-stranded viral DNA | mutant enzyme H250A, at pH 7.5 and 37°C | Homo sapiens |