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Literature summary for 3.5.4.B9 extracted from

  • Chelico, L.; Prochnow, C.; Erie, D.A.; Chen, X.S.; Goodman, M.F.
    Structural model for deoxycytidine deamination mechanisms of the HIV-1 inactivation enzyme APOBEC3G (2010), J. Biol. Chem., 285, 16195-16205.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.B9

Protein Variants

Protein Variants Comment Organism
F126A/W127A site-directed mutagenesis, the N-terminal CD1 domain mutant, that shows disrupted dimerization at the predicted CD1-CD1 dimer interface, predominantly converts Apo3G to a monomer that binds single-stranded DNA, Alu RNA, and catalyzes processive C to U deaminations with 3'-5' deamination polarity, similar to wild-type Apo3G. The mutation causes severe disruption in oligomer formation resulting in about 92% monomers and 8% dimers, with no larger oligomer forms detected Homo sapiens
I314A/Y315A site-directed mutagenesis, C-terminal CD2 domain mutant, C-terminal CD2 domain mutant, mutation at the Apo2 tetrameric interface and predicted CD1 oligomerization region, the mutant contains about 12% tetramers with no larger oligomeric forms Homo sapiens
additional information comparison of Apo3G native and monomeric N-mutant F/W ssDNA substrate binding and catalysis, overview Homo sapiens
R313A/D316A/D317A/Q318A site-directed mutagenesis, C-terminal CD2 domain mutant, mutation at the Apo2 tetrameric interface and predicted CD1 oligomerization region, the mutant contains about 12% tetramers with no larger oligomeric forms Homo sapiens
Y124A/Y125A site-directed mutagenesis, the N-terminal CD1 domain mutant is composed of roughly 47% monomers, 42% dimers, 10% tetramers, and 1% much larger molecular mass species of about 650 kDa Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pre-steady state and steady state kinetics, stopped-flow fluorescence measurements, detailed overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-deoxycytidine + H2O Homo sapiens in ssDNA 2'-deoxyuridine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + H2O in ssDNA Homo sapiens 2'-deoxyuridine + NH3
-
 ?

Subunits

Subunits Comment Organism
More Apo3G has a catalytically inactive N-terminal CD1 domain and an active C-terminal CD2 domain. Apo3G exists as monomers, dimers, tetramers, and higher order oligomers whose distributions depend on DNA substrate and salt Homo sapiens

Synonyms

Synonyms Comment Organism
Apo3G
-
 Homo sapiens
APOBEC3G
-
 Homo sapiens
single-stranded DNA deoxycytidine deaminase
-
 Homo sapiens
single-stranded DNA-dependent deoxycytidine deaminase
-
 Homo sapiens
ssDNA deoxycytidine deaminase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Homo sapiens