Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Thermoplasma acidophilum DSM 1728 |
Crystallization (Comment) | Organism |
---|---|
in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method | Thermoplasma acidophilum DSM 1728 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30600 | - |
estimated from amino acid sequence | Thermoplasma acidophilum DSM 1728 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum DSM 1728 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap affinity column chromatography and Superdex-S200 gel filtration | Thermoplasma acidophilum DSM 1728 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | - |
Thermoplasma acidophilum DSM 1728 | 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Thermoplasma acidophilum DSM 1728 |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase | - |
Thermoplasma acidophilum DSM 1728 |
MTHFDC | - |
Thermoplasma acidophilum DSM 1728 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | preference for NADP+ as a cofactor, rather than NAD+ | Thermoplasma acidophilum DSM 1728 |