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Literature summary for 3.5.4.9 extracted from
- Methenyltetrahydrofolate cyclohydrolase is rate limiting for the enzymatic conversion of 10-formyltetrahydrofolate to 5,10-methylenetetrahydrofolate in bifunctional dehydrogenase-cyclohydrolase enzymes (1998), Biochemistry, 37, 1109-1115.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA encoding bifunctional enzyme cloned and expressed in Escherichia coli K38 | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
10-formyltetrahydrofolate | - |
Photobacterium phosphoreum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mus musculus | 5737 | - |
| cytoplasm | - |
Homo sapiens | 5737 | - |
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Mus musculus |
| 100000 | - |
- |
Sus scrofa |
| 100000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate | Homo sapiens | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | Sus scrofa | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Mus musculus | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Homo sapiens | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Sus scrofa | - |
10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Photobacterium phosphoreum | - |
10-formyltetrahydrofolate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme | - |
| Mus musculus | - |
Ehrlich ascites cells | - |
| Photobacterium phosphoreum | - |
marine bioluminescent bacterium, bifunctional enzyme | - |
| Saccharomyces cerevisiae | - |
yeast, trifunctional enzyme | - |
| Sus scrofa | - |
pig, trifunctional enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate | - |
Homo sapiens | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | - |
Sus scrofa | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Mus musculus | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Sus scrofa | 10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Photobacterium phosphoreum | 10-formyltetrahydrofolate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Saccharomyces cerevisiae |
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