Literature summary for 3.5.4.9 extracted from

Schmidt, A.; Wu, H.; MacKenzie, R.E.; Chen, V.J.; Bewly, J.R.; Ray, J.E.; Toth, J.E; Cygler, M.
Structures of three inhibitor complexes provide insight into the reaction mechanism of the human methylenetetrahydrofolate dehydrogenase/cyclohydrolase (2000), Biochemistry, 39, 6325-6335.

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Application

Application	Comment	Organism
medicine	enzyme represents a potential pharmaceutical target for manipulation of cell growth and development and hence cancer prevention or treatment	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of DC301, the cytosolic DC domain of the trifunctional enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	5,10-methenyltetrahydrofolate	mutant K56R	Homo sapiens
0.32	-	5,10-methenyltetrahydrofolate	mutant S49Q	Homo sapiens
0.34	-	5,10-methenyltetrahydrofolate	wild-type DC301	Homo sapiens
0.48	-	5,10-methenyltetrahydrofolate	mutant Y52F	Homo sapiens
0.52	-	5,10-methenyltetrahydrofolate	mutant S49A	Homo sapiens
0.56	-	5,10-methenyltetrahydrofolate	mutants C147A, T148A	Homo sapiens
1.22	-	5,10-methenyltetrahydrofolate	mutant Y52A	Homo sapiens
1.62	-	5,10-methenyltetrahydrofolate	mutant Y52F	Homo sapiens
2.36	-	5,10-methenyltetrahydrofolate	mutant C147Q	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methenyltetrahydrofolate + H2O	Drosophila melanogaster	-	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	Mus musculus	-	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	Rattus norvegicus	-	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	Homo sapiens	-	10-formyltetrahydrofolate	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-
Homo sapiens	P11586	-	-
Mus musculus	-	-	-
Rattus norvegicus	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.6	-	mutant K56R	Homo sapiens
6.7	-	mutants C147Q, S49Q	Homo sapiens
18	-	mutant C147Q	Homo sapiens
25	-	mutant S49Q	Homo sapiens
39	-	mutant Y52S	Homo sapiens
47	-	mutant Y52A	Homo sapiens
128	-	mutantS49A	Homo sapiens
133	-	DC301, wild-type	Homo sapiens
146	-	mutants C147A, T148A	Homo sapiens
160	-	mutant Y52F	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methenyltetrahydrofolate + H2O	-	Drosophila melanogaster	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	-	Mus musculus	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	-	Rattus norvegicus	10-formyltetrahydrofolate	-	?
5,10-methenyltetrahydrofolate + H2O	-	Homo sapiens	10-formyltetrahydrofolate	-	?

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Release 2023.1 (January 2023)