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Literature summary for 3.5.4.9 extracted from

  • D'Ari, L.; Rabinowitz, J.C.
    Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli (1991), J. Biol. Chem., 266, 23953-23958.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene folD for bifunctional dehydrogenase/cyclohydrolase cloned and sequenced Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-
cytoplasm
-
Homo sapiens 5737
-
cytoplasm
-
Rattus norvegicus 5737
-
cytoplasm
-
Saccharomyces cerevisiae 5737
-
cytoplasm
-
Columba sp. 5737
-
mitochondrion
-
Mus musculus 5739
-
mitochondrion
-
Homo sapiens 5739
-
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31060
-
calculated from amino acid sequence Escherichia coli
35000
-
SDS-PAGE Escherichia coli
70000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O Gallus gallus
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Mus musculus
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Escherichia coli
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Rattus norvegicus
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Sus scrofa
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Oryctolagus cuniculus
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Ovis aries
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Clostridium cylindrosporum
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Moorella thermoacetica
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Columba sp.
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Escherichia coli BE
-
10-formyltetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Clostridium cylindrosporum
-
-
-
Columba sp.
-
pigeon
-
Escherichia coli
-
bifunctional enzyme
-
Escherichia coli BE
-
bifunctional enzyme
-
Gallus gallus
-
chicken, trifunctional enzyme
-
Homo sapiens
-
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme
-
Moorella thermoacetica
-
bifunctional enzyme
-
Mus musculus
-
Ehrlich ascites cells
-
Mus musculus
-
mouse, bifunctional mitochondrial enzyme
-
Oryctolagus cuniculus
-
rabbit
-
Ovis aries
-
sheep, trifunctional enzyme
-
Rattus norvegicus
-
rat, trifunctional cytoplasmic enzyme
-
Saccharomyces cerevisiae
-
yeast, trifunctional enzyme
-
Sus scrofa
-
pig, trifunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-
liver
-
Mus musculus
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
liver
-
Oryctolagus cuniculus
-
liver
-
Ovis aries
-
liver
-
Columba sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C purified enzyme in 50% glycerol, 95% active after 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O
-
Gallus gallus 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Mus musculus 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Escherichia coli 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Homo sapiens 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Rattus norvegicus 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Sus scrofa 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Oryctolagus cuniculus 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Ovis aries 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Clostridium cylindrosporum 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Moorella thermoacetica 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Columba sp. 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Escherichia coli BE 10-formyltetrahydrofolate
-
?

Subunits

Subunits Comment Organism
dimer
-
Saccharomyces cerevisiae
dimer 2 * 35000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
bifunctional enzyme Escherichia coli