Cloned (Comment) | Organism |
---|---|
gene folD for bifunctional dehydrogenase/cyclohydrolase cloned and sequenced | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Rattus norvegicus | 5737 | - |
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
cytoplasm | - |
Columba sp. | 5737 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31060 | - |
calculated from amino acid sequence | Escherichia coli |
35000 | - |
SDS-PAGE | Escherichia coli |
70000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | Gallus gallus | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Mus musculus | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Homo sapiens | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Rattus norvegicus | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Sus scrofa | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Oryctolagus cuniculus | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Ovis aries | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Clostridium cylindrosporum | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Columba sp. | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Escherichia coli BE | - |
10-formyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium cylindrosporum | - |
- |
- |
Columba sp. | - |
pigeon | - |
Escherichia coli | - |
bifunctional enzyme | - |
Escherichia coli BE | - |
bifunctional enzyme | - |
Gallus gallus | - |
chicken, trifunctional enzyme | - |
Homo sapiens | - |
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme | - |
Moorella thermoacetica | - |
bifunctional enzyme | - |
Mus musculus | - |
Ehrlich ascites cells | - |
Mus musculus | - |
mouse, bifunctional mitochondrial enzyme | - |
Oryctolagus cuniculus | - |
rabbit | - |
Ovis aries | - |
sheep, trifunctional enzyme | - |
Rattus norvegicus | - |
rat, trifunctional cytoplasmic enzyme | - |
Saccharomyces cerevisiae | - |
yeast, trifunctional enzyme | - |
Sus scrofa | - |
pig, trifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Mus musculus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
liver | - |
Oryctolagus cuniculus | - |
liver | - |
Ovis aries | - |
liver | - |
Columba sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C purified enzyme in 50% glycerol, 95% active after 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | - |
Gallus gallus | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Mus musculus | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Rattus norvegicus | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Sus scrofa | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Oryctolagus cuniculus | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Ovis aries | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Clostridium cylindrosporum | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Columba sp. | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli BE | 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharomyces cerevisiae |
dimer | 2 * 35000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
bifunctional enzyme | Escherichia coli |