General Stability | Organism |
---|---|
purified enzyme stored in 20% glycerol, 0.1 M potassium phosphate pH 7.3 stable for about 1 week | Sus scrofa |
quite unstable, loses all activity after 96 h of storage | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
5,10-methenyltetrahydrofolate | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
- |
Sus scrofa |
150000 | - |
gel filtration | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | Sus scrofa | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Bos taurus | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Clostridium cylindrosporum | - |
10-formyltetrahydrofolate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | - |
Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
beef | - |
Clostridium cylindrosporum | - |
monofunctional enzyme | - |
Sus scrofa | - |
pig, trifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | - |
Sus scrofa | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Bos taurus | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Clostridium cylindrosporum | 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE, single polypeptide chain | Sus scrofa |