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Literature summary for 3.5.4.9 extracted from

  • Clark, J.E.; Ragsdale, S.W.; Ljungdahl, L.G.; Wiegel, J.
    Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum (1982), J. Bacteriol., 151, 507-509.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O Moorella thermoautotrophica
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Moorella thermoacetica
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Clostridium aceticum
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Clostridium formicaceticum
-
10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O Acetobacterium woodii
-
10-formyltetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Acetobacterium woodii
-
bifunctional enzyme
-
Clostridium aceticum
-
-
-
Clostridium formicaceticum
-
-
-
Moorella thermoacetica
-
-
-
Moorella thermoautotrophica
-
bifunctional enzyme
-
Moorella thermoautotrophica
-
DSM 1974
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.49
-
growth conditions H2 + CO2 Moorella thermoautotrophica
0.63
-
growth conditions glucose + CO2 Moorella thermoautotrophica
0.94
-
growth conditions methanol + CO2 Moorella thermoautotrophica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O
-
Moorella thermoautotrophica 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Moorella thermoacetica 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Clostridium aceticum 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Clostridium formicaceticum 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O
-
Acetobacterium woodii 10-formyltetrahydrofolate
-
?