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Literature summary for 3.5.4.5 extracted from
- Characterization of a novel resistance-related deoxycytidine deaminase from Brassica oleracea var. capitata (2014), J. Agric. Food Chem., 62, 1796-1801.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Brassica oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0913 | - |
2'-deoxycytidine | at pH 7.5 and 27°C | Brassica oleracea |  |
| 1.475 | - |
cytosine beta-D-arabinofuranoside | at pH 7.5 and 27°C | Brassica oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20800 | - |
x * 20800, SDS-PAGE | Brassica oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxycytidine + H2O | Brassica oleracea | - |
2'-deoxyuridine + NH3 | - |
? | |
| cytidine + H2O | Brassica oleracea | - |
uridine + NH3 | - |
? | |
| cytosine beta-D-arabinofuranoside + H2O | Brassica oleracea | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
variant Brassica oleracea capitata | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel chelating affinity column chromatography | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxycytidine + H2O | - |
Brassica oleracea | 2'-deoxyuridine + NH3 | - |
? | |
| cytidine + H2O | - |
Brassica oleracea | uridine + NH3 | - |
? | |
| cytosine beta-D-arabinofuranoside + H2O | - |
Brassica oleracea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20800, SDS-PAGE | Brassica oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DCD | - |
Brassica oleracea |
| deoxycytidine deaminase | - |
Brassica oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
- |
Brassica oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Brassica oleracea |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme plays an important role in the Trichoderma harzianum ETS 323 mediated resistance mechanism in young leaves of Brassice oleracea variant capitata during Rhizoctonia solani infection. The enzyme potentially neutralizes cytotoxic products of host lipoxygenase activity, thereby restricting the hypersensitivity-related programmed cell death induced in plants during the initial stages of infection | Brassica oleracea |
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Release 2023.1 (January 2023)
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