Literature summary for 3.5.4.5 extracted from

Sigmond, J.; Honeywell, R.J.; Postma, T.J.; Dirven, C.M.; de Lange, S.M.; van der Born, K.; Laan, A.C.; Baayen, J.C.; Van Groeningen, C.J.; Bergman, A.M.; Giaccone, G.; Peters, G.J.
Gemcitabine uptake in glioblastoma multiforme: potential as a radiosensitizer (2009), Ann. Oncol., 20, 182-187.

Search for more literature for 3.5.4.5

Application

Application	Comment	Organism
medicine	studies on radiosensitization of glioblastoma multiforme (GBM), deoxynucleoside analogue gemcitabine (dFdC) considered for combination therapy with radiation in glioblastomas	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0092	-	2',2'-difluorodeoxycytidine	i.e. gemcitabine, variation of plasma gemcitabine between 0.9 and 9.2 microM	Homo sapiens
0.0726	-	2',2'-difluorodeoxyuridine	i.e. dFdU, variation of plasma ranges between 24.9 and 72.6 microM	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
deoxycytidine + H2O	Homo sapiens	-	deoxyuridine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	glioblastoma multiforme (GBM) patients	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood plasma	glioblastoma multiforme (GBM) patients	Homo sapiens	-
glioblastoma multiforme cell	glioblastoma multiforme (GBM) patients	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	variation between 1.51 to 5.50 nmol/h/mg protein, about 100-fold lower than that in liver	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2',2'-difluorodeoxycytidine + H2O	deamination of the deoxynucleoside analogue gemcitabine (dFdC) to difluorodeoxyuridine (dFdU), difluorodeoxyuridine used as radiosensitizer in gene therapy	Homo sapiens	2',2'-difluorodeoxyuridine + NH3	-	?
deoxycytidine + H2O	-	Homo sapiens	deoxyuridine + NH3	-	?

Synonyms

Synonyms	Comment	Organism
dCDA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)