Literature summary for 3.5.4.5 extracted from

Teh, A.H.; Kimura, M.; Yamamoto, M.; Tanaka, N.; Yamaguchi, I.; Kumasaka, T.
The 1.48 A resolution crystal structure of the homotetrameric cytidine deaminase from mouse (2006), Biochemistry, 45, 7825-7833.

Cloned(Commentary)

Cloned (Comment)	Organism
enzyme expression in Escherichia coli strain BL21	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with tetrahydrouridine, 3-deazauridine, or cytidine. Two alternate conformations of R68 influence zinc-product interaction	Mus musculus
purified recombinant CDA complexed with either tetrahydrouridine, 3-deazauridine, or cytidine, hanging drop vapor diffusion technique, 25°C, protein solution, containing 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 1 mM DTT, and 5 mM ligand, is mixed with crystallization solution containing 2.0 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, crystals appear after a few hours or several days, respectively, X-ray diffraction structure determination and analysis at 1.48-2.36 A resolutions, molecular replacement	Mus musculus

Crystallization (Comment)

Organism

in complex with tetrahydrouridine, 3-deazauridine, or cytidine. Two alternate conformations of R68 influence zinc-product interaction

Mus musculus

purified recombinant CDA complexed with either tetrahydrouridine, 3-deazauridine, or cytidine, hanging drop vapor diffusion technique, 25°C, protein solution, containing 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 1 mM DTT, and 5 mM ligand, is mixed with crystallization solution containing 2.0 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, crystals appear after a few hours or several days, respectively, X-ray diffraction structure determination and analysis at 1.48-2.36 A resolutions, molecular replacement

Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
3-deazauridine	-	Mus musculus
tetrahydrouridine	-	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent on	Mus musculus
Zn2+	catalytic ion, coordinated by C65, C99, and C102	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2'-deoxycytidine + H2O	Mus musculus	-	2'-deoxyuridine + NH3	-	?
cytidine + H2O	Mus musculus	-	uridine + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P56389	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 by two steps of anion exchange chromatography, and gel filtration	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2'-deoxycytidine + H2O	-	Mus musculus	2'-deoxyuridine + NH3	-	?
cytidine + H2O	-	Mus musculus	uridine + NH3	-	?
cytidine + H2O	cytidine is distinguished from uridine by its 4-NH2 group that acts as a hydrogen bond donor, the charge-neutralizing Arg68 of CDA exhibits two alternate conformations I and II, binding structure, overview	Mus musculus	uridine + NH3	-	?
additional information	active site and substrate binding structure, overview	Mus musculus	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	homotetramer	Mus musculus

Synonyms

Synonyms	Comment	Organism
CDA	-	Mus musculus