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Literature summary for 3.5.4.5 extracted from

  • Sakai, T.; Daikai, T.; Monma, H.; Maeda, H.
    Isolation from Nocardioides sp. strain CT16, purification, and characterization of a deoxycytidine deaminase extremely thermostable in the presence of DL-dithiothreitol (2002), Biosci. Biotechnol. Biochem., 66, 1646-1651.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
dCMP pH 7.0, 37°C Nocardioides sp.
0.13
-
5-methyl-dCMP pH 7.0, 37°C Nocardioides sp.
0.14
-
CMP pH 7.0, 37°C Nocardioides sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
2 * 18000, SDS-PAGE Nocardioides sp.
52000
-
gel filtration Nocardioides sp.

Organism

Organism UniProt Comment Textmining
Nocardioides sp.
-
-
-
Nocardioides sp. CT16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nocardioides sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
225
-
pH 7.0, 37°C Nocardioides sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyl-dCMP + H2O
-
Nocardioides sp. 5-methyl-dCyd
-
?
5-methyl-dCMP + H2O
-
Nocardioides sp. CT16 5-methyl-dCyd
-
?
CMP + H2O
-
Nocardioides sp. ?
-
?
CMP + H2O
-
Nocardioides sp. CT16 ?
-
?
CTP + H2O
-
Nocardioides sp. UTP + NH3
-
?
CTP + H2O
-
Nocardioides sp. CT16 UTP + NH3
-
?
dCMP + H2O
-
Nocardioides sp. ?
-
?
dCMP + H2O
-
Nocardioides sp. CT16 ?
-
?
dCTP + H2O
-
Nocardioides sp. dUTP + NH3
-
?
dCTP + H2O
-
Nocardioides sp. CT16 dUTP + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 18000, SDS-PAGE Nocardioides sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
99
-
maximum of activity Nocardioides sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 min, absence of dithiothreitol, 44% residual activity Nocardioides sp.
100
-
1 h, 1 mM dithiothreitol, 48% residual activity Nocardioides sp.
100
-
1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity Nocardioides sp.
100
-
15 min, 1 mM dithiothreitol, 80% residual activity Nocardioides sp.
100
-
2 h, 50 mM dithiothreitol, 56% residual activity Nocardioides sp.