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Literature summary for 3.5.4.5 extracted from
- Cytidine deaminases (from Escherichia coli and human liver) (1978), Methods Enzymol., 51, 401-407.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,6-Dihydrouridine | competitive inhibition | Escherichia coli |  |
| 5,6-Dihydrouridine | - |
Homo sapiens | |
| uridine | - |
Escherichia coli | |
| uridine | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0092 | - |
cytidine | - |
Homo sapiens | |
| 0.058 | - |
5-azacytidine | - |
Homo sapiens | |
| 0.089 | - |
cytosine deoxyriboside | - |
Escherichia coli | |
| 0.11 | - |
5,6-Dihydrocytidine | - |
Escherichia coli | |
| 0.14 | - |
cytosine arabinoside | - |
Homo sapiens | |
| 0.21 | - |
cytidine | - |
Escherichia coli | |
| 4.2 | - |
6-azacytidine | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
gel filtration, nonhomogenous preparation | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + H2O | Cavia porcellus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Homo sapiens | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Rattus norvegicus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Sus scrofa | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Oryctolagus cuniculus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Canis lupus familiaris | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Macaca mulatta | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Rana sp. | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Columba sp. | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Felis catus domesticus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli B / ATCC 11303 | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
dog | - |
| Cavia porcellus | - |
guinea pig | - |
| Columba sp. | - |
pigeon | - |
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
| Felis catus domesticus | - |
cat | - |
| Homo sapiens | - |
human | - |
| Homo sapiens | - |
HeLa cells | - |
| Macaca mulatta | - |
rhesus monkey | - |
| Mus musculus | - |
mouse | - |
| Mus musculus | - |
BALB/c | - |
| Mus musculus BALB/c | - |
BALB/c | - |
| Oryctolagus cuniculus | - |
rabbit | - |
| Rana sp. | - |
frog | - |
| Rattus norvegicus | - |
rat | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Homo sapiens | - |
| kidney | - |
Mus musculus | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Oryctolagus cuniculus | - |
| liver | - |
Canis lupus familiaris | - |
| liver | - |
Macaca mulatta | - |
| liver | - |
Rana sp. | - |
| liver | - |
Columba sp. | - |
| liver | - |
Felis catus domesticus | - |
| muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, reasonably stable, retains 55% of its activity after 3 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxycytidine + H2O | - |
Mus musculus | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Homo sapiens | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Escherichia coli B / ATCC 11303 | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Mus musculus BALB/c | 2'-deoxyuridine + NH3 | - |
? | |
| 5'-chlorocyclocytidine + H2O | i.e. cytosine, 1beta-D-arabinofuranosyl-2,2'-anhydro-, hydrochloride | Homo sapiens | 5'-chlorocyclouridine + NH3 | - |
? | |
| 5,6-dihydrocytidine + H2O | - |
Escherichia coli | 5,6-dihydrouridine + NH3 | - |
? | |
| 5-azacytidine + H2O | - |
Escherichia coli | 5-azauridine + NH3 | - |
? | |
| 5-azacytidine + H2O | - |
Homo sapiens | 5-azauridine + NH3 | - |
? | |
| 5-bromocytidine + H2O | - |
Homo sapiens | 5-bromouridine + NH3 | - |
? | |
| 5-chlorocytidine + H2O | - |
Homo sapiens | ? + NH3 | - |
? | |
| 5-iodocytidine + H2O | - |
Mus musculus | 5-iodouridine + NH3 | - |
? | |
| 5-iodocytidine + H2O | - |
Homo sapiens | 5-iodouridine + NH3 | - |
? | |
| 5-iodocytidine + H2O | - |
Mus musculus BALB/c | 5-iodouridine + NH3 | - |
? | |
| 6-azacytidine + H2O | - |
Homo sapiens | 6-azauridine + NH3 | - |
? | |
| cytidine + H2O | - |
Cavia porcellus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Rattus norvegicus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Sus scrofa | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Oryctolagus cuniculus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Canis lupus familiaris | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Macaca mulatta | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Rana sp. | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Columba sp. | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Felis catus domesticus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli B / ATCC 11303 | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
| cytosine deoxyriboside + H2O | - |
Escherichia coli | ? | - |
? | |
| deoxycytidine + H2O | - |
Mus musculus | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Homo sapiens | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Canis lupus familiaris | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Escherichia coli B / ATCC 11303 | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Mus musculus BALB/c | deoxyuridine + NH3 | - |
? | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | 3-methylcytidine, cytosine and cyclocytidine are no substrates | Homo sapiens | ? | - |
? | |
| additional information | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
| N4-methylcytidine + H2O | - |
Escherichia coli | uridine + methylamine | - |
r | |
| N4-methylcytidine + H2O | - |
Escherichia coli B / ATCC 11303 | uridine + methylamine | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
identical rates of deamination of cytidine at pH 8 and pH 10 | Homo sapiens |
| 8 | - |
identical rates of deamination of cytidine at pH 5 and pH 10 | Homo sapiens |
| 10 | - |
identical rates of deamination of cytidine at pH 5 and pH 8 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.7 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.7 | irreversible loss of activity at pH values below 4 | Escherichia coli |
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