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Literature summary for 3.5.4.46 extracted from

  • Goble, A.M.; Feng, Y.; Raushel, F.M.; Cronan, J.E.
    Discovery of a cAMP deaminase that quenches cyclic AMP-dependent regulation (2013), ACS Chem. Biol., 8, 2622-2629.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.46

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 3',5'-cyclic AMP pH 7.5, 30°C Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Organism

Organism UniProt Comment Textmining
Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 Q72V44
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cyclic AMP + H2O
-
 Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 3',5'-cyclic IMP + NH3
-
 ?
additional information the enzyme specifically deaminates cAMP to cyclic-3',5'-inosine monophosphate and has no activity on adenosine, adenine, or 5'-adenosine monophosphate Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 ?
-
 ?

Synonyms

Synonyms Comment Organism
ADD
-
 Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
CadD
-
 Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
 3',5'-cyclic AMP pH 7.5, 30°C Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

General Information

General Information Comment Organism
physiological function expression of CadD in Escherichia coli mimics the loss of adenylate cyclase in that it blocks growth on carbon sources that require the cAMP-CRP transcriptional activator complex for expression of the cognate genes. CadD expression represses expression of several cAMP-CRP dependent genes Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
270
-
 3',5'-cyclic AMP pH 7.5, 30°C Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130