Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 5 mM, 30% residual activity, Mn2+, Co2+ or Ni2+ restores | Pseudomonas sp. | |
2-amino-4-hydroxy-1,3,5-s-triazine | i.e, 5-azacytosine, competitive | Pseudomonas sp. | |
8-Hydroxyquinoline-5-sulfonic acid | 5 mM, 30% residual activity, Mn2+, Ni2+ or Co2+ restores | Pseudomonas sp. | |
ammeline | competitive | Pseudomonas sp. | |
EDTA | 5 mM, about 10% loss of activity | Pseudomonas sp. | |
N-ethylammeline | - |
Pseudomonas sp. | |
N-hydroxyethylammeline | competitve | Pseudomonas sp. | |
N-isopropylammeline | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.155 | - |
N-dimethylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
0.299 | - |
N-tert-butylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
0.308 | - |
N-ethylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
0.406 | - |
N-isopropylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
0.58 | - |
N-cyclopropylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
0.817 | - |
N-methylammelide | pH 7.6, 25°C | Pseudomonas sp. | |
1.32 | - |
ammelide | pH 7.6, 25°C | Pseudomonas sp. | |
3.86 | - |
N-hydroxyethylammelide | pH 7.6, 25°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | completely restores activity of metal-depleted enzyme | Pseudomonas sp. | |
Mn2+ | completely restores activity of metal-depleted enzyme | Pseudomonas sp. | |
Ni2+ | completely restores activity of metal-depleted enzyme | Pseudomonas sp. | |
Zn2+ | 0.5 mol per mol of subunit | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence | Pseudomonas sp. |
44900 | - |
4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence | Pseudomonas sp. |
174000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas sp. |
Renatured (Comment) | Organism |
---|---|
activity of metal-depleted enzyme may be restored by Zn2+, Fe2+, Mn2+, Co2+, Ni2+ | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.2 | - |
25°C, pH 7.6 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + NH3 | - |
? | |
N-cyclopropylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + cyclopropylamine | - |
? | |
N-dimethylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + dimethylamine | - |
? | |
N-ethylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + ethylamine | - |
? | |
N-hydroxyethylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + 2-aminoethanol | - |
? | |
N-isopropylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + isopropylamine | - |
? | |
N-methylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + methylamine | - |
? | |
N-tert-butylammelide + H2O | - |
Pseudomonas sp. | cyanuric acid + tert-butylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE, 4 * 44900, deduced from gene sequence | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
2-amino-4-hydroxy-1,3,5-s-triazine | pH 7.6, 25°C | Pseudomonas sp. | |
0.129 | - |
N-hydroxyethylammeline | pH 7.6, 25°C | Pseudomonas sp. | |
0.687 | - |
ammeline | pH 7.6, 25°C | Pseudomonas sp. |