Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli S Phi 3834 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Homo sapiens | |
N6-methyladenosine | - |
Homo sapiens | |
purine riboside | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
adenosine | 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
SDS-PAGE, calculated value 40754 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | Homo sapiens | - |
inosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity, protamine sulfate treatment, Q Sepharose chromatography, purine riboside-Sepharose chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
protamine sulfate supernatant | Homo sapiens |
52 | - |
Q Sepharose chromatography | Homo sapiens |
260 | - |
purine riboside Sepharose chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Homo sapiens | inosine + NH3 | - |
? | |
adenosine + H2O | adenosine deaminase markedly enhances the agonist and antagonist affinity and abolishes the negative cooperativity on agonist binding to human striatial adenosine A1 receptors | Homo sapiens | inosine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Homo sapiens |
Adenosine aminohydrolase | - |
Homo sapiens |
adenosine deaminase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
180 | - |
adenosine | 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
purine riboside | 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM | Homo sapiens | |
0.018 | - |
N6-methyladenosine | 25°C, 50 mM Tris-HCl buffer, pH 7.4, enzyme concentration 1.5 nM | Homo sapiens |