Literature summary for 3.5.4.4 extracted from

Ajloo, D.; Taghizadeh, E.; Saboury, A.A.; Bazyari, E.; Mahnam, K.
Effects of surfactant, salt and solvent on the structure and activity of adenosine deaminase: molecular dynamic and spectrophotometric studies (2008), Int. J. Biol. Macromol., 43, 151-158.

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Protein Variants

Protein Variants	Comment	Organism
additional information	effects of sodium dodecyl sulfate, dodecyltrimethylammonium bromide, sodium chloride, sodium sulfate, methanol and ethanol, on the structure and activity of adenosine deaminase are investigated by UV-Vis, circular dichroism spectrophotometry and molecular dynamics studies. Relative activity, experimental and computational helix content, total accessible surface area and exposed charged surface area are obtained. The relative activity of adenosine deaminase in the absence and the presence of denaturants is compared with structural results. It is shown that an increase in the surface area and a decrease in the amount of helicity are associated with a decrease in the activity of ADA	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
dodecyltrimethylammonium bromide	at low concentrations (approximately 0.1 mM), enzyme activity decreases, it increases at a slightly higher concentration (about 0.2 mM), and finally decreases again (about 0.3 mM), CD spectra of adenosine deaminase and DTAB, determination of helix content, MD calculations	Bos taurus
ethanol	CD spectra of adenosine deaminase and EtOH, determination of helix content, MD calculations	Bos taurus
methanol	MD calculations, discussion of effects	Bos taurus
additional information	the trend of relative activity is directly proportional to helicity and inversely proportional to accessible surface area	Bos taurus
Na2SO4	CD spectra of adenosine deaminase and Na2SO4, determination of helix content, MD calculations, discussion of effects	Bos taurus
NaCl	MD calculations, discussion of effects	Bos taurus
Sodium dodecyl sulfate	at low concentrations (approximately 0.1 mM) enzyme activity decreases, it increases at a slightly higher concentration (about 0.4 mM), and finally decreases again (about 0.55 mM), CD spectra of adenosine deaminase and SDS, determination of helix content, MD calculations	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P56658	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestinal mucosa	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenosine + H2O	-	Bos taurus	inosine + NH3	-	?

Synonyms

Synonyms	Comment	Organism
ADA	-	Bos taurus
adenosine deaminase	-	Bos taurus

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Release 2023.1 (January 2023)