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Literature summary for 3.5.4.38 extracted from

  • Larijani, M.;Petrov, A.P.; Kolenchenko, O.; Berru, M.; Krylov, S.N.; Martin, A.
    AID associates with single-stranded DNA with high affinity and a long complex half-life in a sequence-independent manner (2007), Mol. Cell. Biol., 27, 20-30.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a glutathione S-transferase fusion protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km of glutathione S-transferase-AID for the hot-spot transcription bubble substrate HS1bub7 is between 10 and 15 nM. It is not possible to estimate the Km for the cold-spot bubble (i.e., CS1bub7) since the substrate saturation kinetics is not observed. However, as the velocity continues to increase beyond the highest substrate concentration tested, the Km is likely to be severalfold higher for CS1bub7 than for HS1bub7 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine in single-stranded DNA + H2O Homo sapiens
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uracil in single-stranded DNA + NH3
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9GZX7
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-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine in single-stranded DNA + H2O
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Homo sapiens uracil in single-stranded DNA + NH3
-
?
cytosine in single-stranded DNA + H2O activation-induced cytidine deaminase initiates secondary antibody diversification processes by deaminating cytidines on single-stranded DNA. AID preferentially deaminates cytidines preceded by W(A/T)R(A/G) dinucleotides, a sequence specificity that is evolutionarily conserved from bony fish to humans. AID preferentially deaminates bubble substrates of five to seven nucleotides rather than larger bubbles and preferentially binds to bubble-type rather than to single-stranded DNA substrates, suggesting that the natural targets of AID are either transcription bubbles or stem-loop structures. AID displays high affinity for single-stranded DNA. All substrates used in activity assays contain a single cytidine within the bubble and differ in the dinucleotide immediately upstream of the target cytidine constituting either WRC motifs (hot spots) or non-WRC motifs (cold spots) Homo sapiens uracil in single-stranded DNA + NH3
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
AID
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens