Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a glutathione S-transferase fusion protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km of glutathione S-transferase-AID for the hot-spot transcription bubble substrate HS1bub7 is between 10 and 15 nM. It is not possible to estimate the Km for the cold-spot bubble (i.e., CS1bub7) since the substrate saturation kinetics is not observed. However, as the velocity continues to increase beyond the highest substrate concentration tested, the Km is likely to be severalfold higher for CS1bub7 than for HS1bub7 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded DNA + H2O | Homo sapiens | - |
uracil in single-stranded DNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9GZX7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded DNA + H2O | - |
Homo sapiens | uracil in single-stranded DNA + NH3 | - |
? | |
cytosine in single-stranded DNA + H2O | activation-induced cytidine deaminase initiates secondary antibody diversification processes by deaminating cytidines on single-stranded DNA. AID preferentially deaminates cytidines preceded by W(A/T)R(A/G) dinucleotides, a sequence specificity that is evolutionarily conserved from bony fish to humans. AID preferentially deaminates bubble substrates of five to seven nucleotides rather than larger bubbles and preferentially binds to bubble-type rather than to single-stranded DNA substrates, suggesting that the natural targets of AID are either transcription bubbles or stem-loop structures. AID displays high affinity for single-stranded DNA. All substrates used in activity assays contain a single cytidine within the bubble and differ in the dinucleotide immediately upstream of the target cytidine constituting either WRC motifs (hot spots) or non-WRC motifs (cold spots) | Homo sapiens | uracil in single-stranded DNA + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
AID | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |