Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium acetate | 0.4 M, complete inhibition | Xenopus laevis | |
Basic proteins | histones and lysozyme, 0.5 mg/ml, complete inhibition | Xenopus laevis | |
Double-stranded RNA | excess of substrate inhibits | Xenopus laevis | |
heparin | 0.5 mg/ml, complete inhibition | Xenopus laevis | |
iodoacetate | 10 mM, complete inhibition | Xenopus laevis | |
KCl | 0.2 M, complete inhibition | Xenopus laevis | |
additional information | no inhibition: DNA (salmon sperm, 0.3 mg/ml), RNA (torula yeast, 0.3 mg/ml), acidic proteins (bovine serum albumin and casein, 2.0 mg/ml), polyglutamic acid (2.0 mg/ml), glutamic acid (2 mM), glutamine (2 mM), glyceraldehyde 6-phosphate (2 mM), CaCl2 (5.0 mM), MgCl2 (5.0 mM), EDTA (10 mM), EGTA (10 mM), ATP (10 mM), GTP (10 mM), 5'-AMP (10 mM), adenosine (10 mM), hypoxanthine (10 mM) | Xenopus laevis | |
N-ethylmaleimide | 10 mM, complete inhibition | Xenopus laevis | |
NaCl | 0.2 M, complete inhibition | Xenopus laevis | |
potassium phosphate | - |
Xenopus laevis | |
Sodium phosphate | - |
Xenopus laevis | |
spermidine | 0.5 mg/ml, complete inhibition | Xenopus laevis | |
TLCK | 1 mM, complete inhibition | Xenopus laevis | |
ZnCl2 | 1 mM, complete inhibition | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE | Xenopus laevis |
120000 | - |
gel filtration | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine in double-stranded RNA + H2O | Xenopus laevis | - |
hypoxanthine in double-stranded RNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
25°C, pH 7.9 | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine in double-stranded RNA + H2O | - |
Xenopus laevis | hypoxanthine in double-stranded RNA + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
double-stranded RNA adenosine deaminase | - |
Xenopus laevis |
dsRAD | - |
Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | Tris or phosphate buffer | Xenopus laevis |