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Literature summary for 3.5.4.37 extracted from

  • Hough, R.F.; Bass, B.L.
    Purification of the Xenopus laevis double-stranded RNA adenosine deaminase (1994), J. Biol. Chem., 269, 9933-9939.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ammonium acetate 0.4 M, complete inhibition Xenopus laevis
Basic proteins histones and lysozyme, 0.5 mg/ml, complete inhibition Xenopus laevis
Double-stranded RNA excess of substrate inhibits Xenopus laevis
heparin 0.5 mg/ml, complete inhibition Xenopus laevis
iodoacetate 10 mM, complete inhibition Xenopus laevis
KCl 0.2 M, complete inhibition Xenopus laevis
additional information no inhibition: DNA (salmon sperm, 0.3 mg/ml), RNA (torula yeast, 0.3 mg/ml), acidic proteins (bovine serum albumin and casein, 2.0 mg/ml), polyglutamic acid (2.0 mg/ml), glutamic acid (2 mM), glutamine (2 mM), glyceraldehyde 6-phosphate (2 mM), CaCl2 (5.0 mM), MgCl2 (5.0 mM), EDTA (10 mM), EGTA (10 mM), ATP (10 mM), GTP (10 mM), 5'-AMP (10 mM), adenosine (10 mM), hypoxanthine (10 mM) Xenopus laevis
N-ethylmaleimide 10 mM, complete inhibition Xenopus laevis
NaCl 0.2 M, complete inhibition Xenopus laevis
potassium phosphate
-
Xenopus laevis
Sodium phosphate
-
Xenopus laevis
spermidine 0.5 mg/ml, complete inhibition Xenopus laevis
TLCK 1 mM, complete inhibition Xenopus laevis
ZnCl2 1 mM, complete inhibition Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE Xenopus laevis
120000
-
gel filtration Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine in double-stranded RNA + H2O Xenopus laevis
-
hypoxanthine in double-stranded RNA + NH3
-
?

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
25°C, pH 7.9 Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine in double-stranded RNA + H2O
-
Xenopus laevis hypoxanthine in double-stranded RNA + NH3
-
?

Subunits

Subunits Comment Organism
? x * 12000, the purified monomeric enzyme form is active, but it is not known if the enzyme functions strictly as a monomer, it may dimerize in the presence of substrate or associate in crude mixture with other cellular constituents, SDS-PAGE Xenopus laevis

Synonyms

Synonyms Comment Organism
double-stranded RNA adenosine deaminase
-
Xenopus laevis
dsRAD
-
Xenopus laevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8.5 Tris or phosphate buffer Xenopus laevis