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Literature summary for 3.5.4.36 extracted from

  • Chester, A.; Weinreb, V.; Carter, C.W.Jr.; Navaratnam, N.
    Optimization of apolipoprotein B mRNA editing by APOBEC1 apoenzyme and the role of its auxiliary factor, ACF (2004), RNA, 10, 1399-1411.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
APOBEC1 complementation factor broadens the temperature range of APOBEC1 activity, lowers the optimal temperature and enables it to function optimally at lower temperatures, it promotes at lower temperatures a conformational transition in the RNA substrate that occurs spontaneously at higher temperature Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
high level expression of APOBEC1 in Sf9 cells as an N-terminal GST fusion protein. Substantial RNA editing activity of APOBEC1 alone may be responsible for the hyperediting observed upon overexpression of APOBEC1 in transgenic mice. These animals develop hepatocellular carcinomas Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000017
-
cytosine6666 in apolipoprotein B mRNA 30°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF Homo sapiens
0.000000027
-
cytosine6666 in apolipoprotein B mRNA 41°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF Homo sapiens
0.00000021
-
cytosine6666 in apolipoprotein B mRNA 30°C, pH 7.5, APOBEC1 without auxiliary factor Homo sapiens
0.00000022
-
cytosine6666 in apolipoprotein B mRNA 41°C, pH 7.5, APOBEC1 without auxiliary factor Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl optimal activity of APOBEC1 without auxiliary factor at 80 mM ionic strength, optimal activity of APOBEC1 in presence of the auxiliary factor ACF at 90 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine6666 in apolipoprotein B mRNA + H2O Homo sapiens
-
uracil6666 in apolipoprotein B mRNA + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P41238
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine6666 in apolipoprotein B mRNA + H2O
-
Homo sapiens uracil6666 in apolipoprotein B mRNA + NH3
-
?
cytosine6666 in apolipoprotein B mRNA + H2O the apolipoprotein B mRNA editing subunit, APOBEC1, has considerable residual enzymatic activity on a minimal apoB mRNA substrate, even in the absence of any auxiliary factors. The effect of the auxiliary factor ACF is to broaden the temperature range of APOBEC1 activity, lowering the optimal temperature and enabling it to function optimally at lower temperatures. A model consistent with this observation is that at lower temperatures ACF promotes a conformational transition in the RNA substrate that occurs spontaneously at higher temperature Homo sapiens uracil6666 in apolipoprotein B mRNA + NH3
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
APOBEC1 catalytic component of an RNA-editing complex Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
APOBEC1 in presence of the auxiliary factor ACF Homo sapiens
45
-
APOBEC1 without auxiliary factor Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
APOBEC1 in presence of the auxiliary factor ACF Homo sapiens
7.5
-
APOBEC1 without auxiliary factor Homo sapiens