Activating Compound | Comment | Organism | Structure |
---|---|---|---|
APOBEC1 complementation factor | broadens the temperature range of APOBEC1 activity, lowers the optimal temperature and enables it to function optimally at lower temperatures, it promotes at lower temperatures a conformational transition in the RNA substrate that occurs spontaneously at higher temperature | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
high level expression of APOBEC1 in Sf9 cells as an N-terminal GST fusion protein. Substantial RNA editing activity of APOBEC1 alone may be responsible for the hyperediting observed upon overexpression of APOBEC1 in transgenic mice. These animals develop hepatocellular carcinomas | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000017 | - |
cytosine6666 in apolipoprotein B mRNA | 30°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF | Homo sapiens | |
0.000000027 | - |
cytosine6666 in apolipoprotein B mRNA | 41°C, pH 7.0, APOBEC1 in presence of the auxiliary factor ACF | Homo sapiens | |
0.00000021 | - |
cytosine6666 in apolipoprotein B mRNA | 30°C, pH 7.5, APOBEC1 without auxiliary factor | Homo sapiens | |
0.00000022 | - |
cytosine6666 in apolipoprotein B mRNA | 41°C, pH 7.5, APOBEC1 without auxiliary factor | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | optimal activity of APOBEC1 without auxiliary factor at 80 mM ionic strength, optimal activity of APOBEC1 in presence of the auxiliary factor ACF at 90 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine6666 in apolipoprotein B mRNA + H2O | Homo sapiens | - |
uracil6666 in apolipoprotein B mRNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41238 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine6666 in apolipoprotein B mRNA + H2O | - |
Homo sapiens | uracil6666 in apolipoprotein B mRNA + NH3 | - |
? | |
cytosine6666 in apolipoprotein B mRNA + H2O | the apolipoprotein B mRNA editing subunit, APOBEC1, has considerable residual enzymatic activity on a minimal apoB mRNA substrate, even in the absence of any auxiliary factors. The effect of the auxiliary factor ACF is to broaden the temperature range of APOBEC1 activity, lowering the optimal temperature and enabling it to function optimally at lower temperatures. A model consistent with this observation is that at lower temperatures ACF promotes a conformational transition in the RNA substrate that occurs spontaneously at higher temperature | Homo sapiens | uracil6666 in apolipoprotein B mRNA + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
APOBEC1 | catalytic component of an RNA-editing complex | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
APOBEC1 in presence of the auxiliary factor ACF | Homo sapiens |
45 | - |
APOBEC1 without auxiliary factor | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
APOBEC1 in presence of the auxiliary factor ACF | Homo sapiens |
7.5 | - |
APOBEC1 without auxiliary factor | Homo sapiens |