Literature summary for 3.5.4.35 extracted from

Randau, L.; Stanley, B.; Kohlway, A.; Mechta, S.; Xiong, Y.; Soell, D.
A cytidine deaminase edits C to U in transfer RNAs in archaea (2009), Science, 324, 657-659.

Search for more literature for 3.5.4.35

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methanopyrus kandleri

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion. The structure of the enzyme is solved in two different crystal forms. In each form, the asymmetric unit is composed of two identical CDAT8 dimers	Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30600	-	2 * 30600	Methanopyrus kandleri
60000	-	gel filtration	Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytosine8 in tRNA + H2O	Methanopyrus kandleri	all canonical tRNAs have a uridine at position 8, involved in maintaining tRNA tertiary structure. The hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. The enzyme catalyzes C-to-U editing at this location. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all Methanopyrus kandleri tRNAs	uracil8 in tRNA + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanopyrus kandleri	Q8TWU6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cytosine8 in tRNA + H2O	all canonical tRNAs have a uridine at position 8, involved in maintaining tRNA tertiary structure. The hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. The enzyme catalyzes C-to-U editing at this location. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all Methanopyrus kandleri tRNAs	Methanopyrus kandleri	uracil8 in tRNA + NH3	-	?
cytosine8 in tRNA + H2O	the enzyme is specific for C deamination at position 8, requires only the acceptor stem hairpin for activity. CDAT8 is able to recognize 30 different tRNAs mainly by interacting with the tRNA acceptor stem, including the universal 3'-CCA end, and a flexible base C8	Methanopyrus kandleri	uracil8 in tRNA + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30600	Methanopyrus kandleri

Synonyms

Synonyms	Comment	Organism
CDAT8	-	Methanopyrus kandleri
MK0935	-	Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	assay at	Methanopyrus kandleri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	assay at	Methanopyrus kandleri

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)