Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Halalkalibacterium halodurans |
Crystallization (Comment) | Organism |
---|---|
in complex with dTTP, hanging drop vapor diffusion method, using 200 mM lithium citrate and 20% (w/v) polyethylene glycol 4000 | Halalkalibacterium halodurans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | - |
Halalkalibacterium halodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + H2O | Halalkalibacterium halodurans | - |
dUMP + diphosphate + NH3 | - |
? | |
dCTP + H2O | Halalkalibacterium halodurans C-125 | - |
dUMP + diphosphate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | Q9KFV3 | - |
- |
Halalkalibacterium halodurans C-125 | Q9KFV3 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DE-52 anion-exchange column chromatography | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCTP + H2O | - |
Halalkalibacterium halodurans | dUMP + diphosphate + NH3 | - |
? | |
dCTP + H2O | - |
Halalkalibacterium halodurans C-125 | dUMP + diphosphate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | x-ray crystallography | Halalkalibacterium halodurans |
Synonyms | Comment | Organism |
---|---|---|
DCD:DUT | bifunctional enzyme with dCTP deaminase and dUTPase activities | Halalkalibacterium halodurans |
dcdB | - |
Halalkalibacterium halodurans |